Identification of three histone methyltransferases in Drosophila: dG9a is a suppressor of PEV and is required for gene silencing.

Molecular Genetics and Genomics : MGG
Jacek MisThomas A Grigliatti

Abstract

Organization of chromatin structure and regulation of gene transcription are contingent on histone tail modifications. Regions of the genome packaged with nucleosomes that contain methyl histone H3 at lysine 9 (Me K9H3) strongly correlate with regions that are silenced for transcription. To date Su(var)3-9 is the only K9H3 specific enzyme characterized in Drosophila melanogaster. In this study, we describe the identification of three additional Drosophila genes that potentially encode K9H3 specific methyltransferases (HMTase) with homology to known mammalian proteins. By several criteria, including sequence alignments, phylogenic analyses, and enzyme activity of the protein, one of these is a homologue of the human G9a and hence, we name it dG9a. dG9a catalyzes the transfer of methyl groups to full-length histone H3 and to N-terminal H3 peptides that contain lysine 9, suggesting that the major target for dG9a is K9H3. Chromatin extracts prepared from a P-element insert mutation in dG9a display an altered K9H3 methylation profile. In addition, the dG9a mutant is a dominant suppressor of position-effect variegation (PEV), a heterochromatin-associated gene silencing phenomenon. Su(var)3-9 also suppresses PEV. The combined Su(var)3...Continue Reading

References

Sep 1, 1977·Nature·J T FinchA Klug
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L F AppelJ Fristrom
Feb 1, 1997·Trends in Biochemical Sciences·C P Ponting
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·A R MushegianE V Koonin
Mar 6, 1998·Cellular and Molecular Life Sciences : CMLS·T JenuweinG Reuter
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·B D StrahlC D Allis
Jan 19, 2000·Nature·B D Strahl, C D Allis
Aug 11, 2001·Science·T Jenuwein, C D Allis
Aug 22, 2002·Nature Reviews. Cancer·Shi Huang
Oct 10, 2002·Cell·Raymond C TrievelJames H Hurley
Oct 22, 2002·Nature Structural Biology·Jinrong MinRui-Ming Xu
Mar 22, 2003·Current Opinion in Cell Biology·Wolfgang FischleC David Allis
May 6, 2003·Journal of Cell Science·Monika LachnerThomas Jenuwein
Jul 8, 2003·Science of Aging Knowledge Environment : SAGE KE·Alejandro VaqueroDanny Reinberg
Jul 31, 2003·Molecular Cell·Xing ZhangXiaodong Cheng
Dec 17, 2003·Current Opinion in Structural Biology·Bing XiaoSteven J Gamblin
Dec 24, 2003·Molecular Cell·Antoine H F M PetersThomas Jenuwein
Feb 7, 2004·The EMBO Journal·Slimane Ait-Si-AliAnnick Harel-Bellan
Feb 27, 2004·Nature Immunology·Ildikó GyoryKenneth L Wright
May 18, 2004·Genes & Development·Gunnar SchottaThomas Jenuwein
Jul 23, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hitomi Nishio, Martin J Walsh
Jan 1, 1950·Advances in Genetics·E B LEWIS
Nov 30, 2004·Science·Benedetta DorigoTimothy J Richmond
Jun 9, 2005·Nucleic Acids Research·Pierre-Olivier EstèveSriharsa Pradhan

❮ Previous
Next ❯

Citations

Oct 24, 2013·Trends in Biochemical Sciences·Hans-Martin HerzAli Shilatifard
Sep 12, 2006·Nucleic Acids Research·Marianne StabellAndrew Lambertsson
Apr 23, 2011·Cell Structure and Function·Yasuko KatoMasamitsu Yamaguchi
Jan 20, 2011·PLoS Biology·Jamie M KramerAnnette Schenck
May 16, 2007·PLoS Genetics·Carole SeumSéverine Bontron
May 6, 2008·PLoS Genetics·Kazuma HanaiSusumu Hirose
Dec 6, 2008·PloS One·J Greg DohenyThomas A Grigliatti
Nov 28, 2007·Genetics·Carole SeumPierre Spierer
Sep 16, 2008·Biochimica Et Biophysica Acta·Kathryn L Huisinga, Sarah C R Elgin
Sep 4, 2015·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kouhei ShimajiMasamitsu Yamaguchi
May 24, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yasuko KatoMasamitsu Yamaguchi
Dec 17, 2009·Insect Molecular Biology·K-S LeeY-K Kang
Sep 18, 2012·Biochimica Et Biophysica Acta·Michaela Smolle, Jerry L Workman
Aug 23, 2011·Current Biology : CB·David J Finnegan
Aug 11, 2017·Neuroscience Bulletin·Yao TianJunhai Han
Oct 11, 2019·Archives of Insect Biochemistry and Physiology·Lei YangGongyin Ye
Mar 17, 2007·Hereditas·Marianne StabellAndrew Lambertsson
Jan 31, 2018·Genetics·Ryan J GleasonXin Chen
Aug 28, 2016·Genes & Development·Taylor J R PenkeRobert J Duronio
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tsai-Yu TzengC-K James Shen
Feb 28, 2019·Genes & Development·Charles A SellerPatrick H O'Farrell
Dec 9, 2020·Journal of Cell Science·Darya A KalashnikovaDmitry E Koryakov
Dec 14, 2006·Development·Emily CloughTulle Hazelrigg
Apr 7, 2018·Mechanisms of Ageing and Development·Ho-Jun JeonMi-Ae Yoo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.