Identification of Three lncRNAs as Potential Predictive Biomarkers of Lung Adenocarcinoma

BioMed Research International
Donghui JinPeng Zhang

Abstract

Lung cancer is the most common cancer and the most common cause of cancer-related death worldwide. However, the molecular mechanism of its development is unclear. It is imperative to identify more novel biomarkers. Two datasets (GSE70880 and GSE113852) were downloaded from the Gene Expression Omnibus (GEO) database and used to identify the differentially expressed genes (DEGs) between lung cancer tissues and normal tissues. Then, we constructed a competing endogenous RNA (ceRNA) network and a protein-protein interaction (PPI) network and performed gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and survival analyses to identify potential biomarkers that are related to the diagnosis and prognosis of lung cancer. A total of 41 lncRNAs and 805 mRNAs were differentially expressed in lung cancer. The ceRNA network contained four lncRNAs (CLDN10-AS1, SFTA1P, SRGAP3-AS2, and ADAMTS9-AS2), 21 miRNAs, and 48 mRNAs. Functional analyses revealed that the genes in the ceRNA network were mainly enriched in cell migration, transmembrane receptor, and protein kinase activity. mRNAs DLGAP5, E2F7, MCM7, RACGAP1, and RRM2 had the highest connectivity in the PPI network. Immunohistochemistry (IHC) dem...Continue Reading

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Citations

Apr 27, 2021·Cancer Biomarkers : Section a of Disease Markers·Yuxin ZhaoZhihua Yin
Aug 3, 2021·Frontiers in Oncology·Lisa SuXiangdong Kong

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Datasets Mentioned

BETA
GSE113852
GSE70880
ADAMTS9-AS2

Software Mentioned

clusterprofiler
Cytoscape
STRING
limma
sva
R
GraphPad Prism

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