Identification of three proteins that associate in vitro with the Leishmania (Leishmania) amazonensis G-rich telomeric strand

European Journal of Biochemistry
Maribel F FernándezM I N Cano

Abstract

The chromosomal ends of Leishmania (Leishmania) amazonensis contain conserved 5'-TTAGGG-3' telomeric repeats. Protein complexes that associate in vitro with these DNA sequences, Leishmania amazonensis G-strand telomeric protein (LaGT1-3), were identified and characterized by electrophoretic mobility shift assays and UV cross-linking using protein fractions purified from S100 and nuclear extracts. The three complexes did not form (a) with double-stranded DNA and the C-rich telomeric strand, (b) in competition assays using specific telomeric DNA oligonucleotides, or (c) after pretreatment with proteinase K. LaGT1 was the most specific and did not bind a Tetrahymena telomeric sequence. All three LaGTs associated with an RNA sequence cognate to the telomeric G-rich strand and a complex similar to LaGT1 is formed with a double-stranded DNA bearing a 3' G-overhang tail. The protein components of LaGT2 and LaGT3 were purified by affinity chromatography and identified, after renaturation, as approximately 35 and approximately 52 kDa bands, respectively. The <or= 15 kDa protein component of LaGT1 was gel-purified as a UV cross-linked complex of approximately 18-20 kDa. Peptides generated from trypsin digestion of the affinity and gel-pu...Continue Reading

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Citations

Jul 1, 2006·Molecular and Cellular Biology·Beiyu LiuPaul T Englund
Jun 21, 2007·Trends in Parasitology·Cristina B B LiraMaria Isabel N Cano
May 18, 2007·Biochemical and Biophysical Research Communications·C B B LiraM I N Cano
May 15, 2007·Biochemical and Biophysical Research Communications·J L Siqueira NetoM I N Cano
Aug 30, 2005·International Journal for Parasitology·F F Conte, M I N Cano
Aug 17, 2014·Biochemical and Biophysical Research Communications·Na ZhaoXichen Zhang
Oct 19, 2017·The Journal of Eukaryotic Microbiology·Raphael Souza PavaniMaria Carolina Elias
Mar 31, 2020·Biochimica Et Biophysica Acta. General Subjects·Carlos A H FernandesMaria Isabel Nogueira Cano

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