Identification of tocopherol (vitamin E) in the skin and its histochemical detection

Acta histochemica
W A Hadler, S R Silveira

Abstract

The epidermis upper layer reacts positively to the performic acid-toluidine blue and the performic acid-Schiff; such reactions were inhibited by a previous formalin-HgCl2 fixation. The substance accounted for that reactivity could be extracted from skin histological sections by means of the chloroform-methanol-HCl effect. If this same solvent was used on human skin shaven horny layer powder it was able to extract protein-lipid complex that displays the tocopherol histochemical reactivity. Since this complex was subjected to the KOH hydrolysis, an hexane-soluble lipid moiety could be isolated from the hexane-insoluble protein moiety. The isolated lipid moiety react as does the tocopherol histochemically, in opposite to the protein moiety that react negatively to the performic acid-toluidine blue and the performic acid-Schiff reactions. By using thin layer chromatography it was possible to isolate from the lipid moiety two substances identified as alpha-tocopherol and cholesterol in basis of its chromatographic properties, the first being the sole responsible by the performic acid-toluidine blue and performic acid-Schiff reactivity of the lipid moiety. From the other fractions isolated from the horny layer powder, either the lipi...Continue Reading

References

Jan 1, 1981·Acta histochemica·W A Hadler, S R Silveira
Mar 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R D LILLIEE R FISHER
Jul 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R D LILLIE, R BANGLE
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Jul 1, 1956·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B H LANDING, H E HALL
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Jul 1, 1960·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C W ADAMS
Nov 1, 1961·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C JOBST, A HORVATH
Aug 30, 1947·Nature·F SANGER

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Citations

Jan 1, 1985·Acta histochemica·S R Silveira, W A Hadler

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