Identification of TOEFAZ1-interacting proteins reveals key regulators of Trypanosoma brucei cytokinesis

Molecular Microbiology
Nicholas A HiltonChristopher L de Graffenried

Abstract

The protist parasite Trypanosoma brucei is an obligate extracellular pathogen that retains its highly polarized morphology during cell division and has evolved a novel cytokinetic process independent of non-muscle myosin II. The polo-like kinase homolog TbPLK is essential for transmission of cell polarity during division and for cytokinesis. We previously identified a putative TbPLK substrate named Tip of the Extending FAZ 1 (TOEFAZ1) as an essential kinetoplastid-specific component of the T. brucei cytokinetic machinery. We performed a proximity-dependent biotinylation identification (BioID) screen using TOEFAZ1 as a means to identify additional proteins that are involved in cytokinesis. Using quantitative proteomic methods, we identified nearly 500 TOEFAZ1-proximal proteins and characterized 59 in further detail. Among the candidates, we identified an essential putative phosphatase that regulates the expression level and localization of both TOEFAZ1 and TbPLK, a previously uncharacterized protein that is necessary for the assembly of a new cell posterior, and a microtubule plus-end directed orphan kinesin that is required for completing cleavage furrow ingression. The identification of these proteins provides new insight into...Continue Reading

References

Jan 1, 1988·Cell Motility and the Cytoskeleton·J M GalloJ Schrével
Jan 1, 1989·Cell Motility and the Cytoskeleton·A von MassowE Mandelkow
Jan 1, 1988·Biology of the Cell·J M Gallo, E Precigout
Jun 1, 1982·The Journal of Cell Biology·J V KilmartinC Milstein
Mar 1, 1993·Molecular and Biochemical Parasitology·L RindisbacherT Seebeck
Feb 21, 1998·Nucleic Acids Research·E L SonnhammerR Durbin
Jan 24, 1998·Biochemistry·S SackE Mandelkow
Nov 5, 1999·Annual Review of Microbiology·K Gull
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·G Woehlke, M Schliwa
Mar 6, 2002·The Journal of Biological Chemistry·Douglas J LaCountJohn E Donelson
Mar 22, 2002·Molecular Biology of the Cell·Cécile VedrenneThéo Baltz
Apr 23, 2003·Molecular and Biochemical Parasitology·Seth RedmondMark C Field
Oct 9, 2003·The EMBO Journal·Linda KohlPhilippe Bastin
Feb 14, 2004·The EMBO Journal·José A García-SalcedoDerek P Nolan
Feb 22, 2005·Methods : a Companion to Methods in Enzymology·Claire Delahunty, John R Yates
Aug 27, 2005·Nature·Thomas A Richards, Thomas Cavalier-Smith
Dec 1, 2005·Bioinformatics·A V McDonnellB Berger
Jan 10, 2006·Eukaryotic Cell·Praveen Kumar, C C Wang
Feb 17, 2006·Molecular Biology of the Cell·Bill Wickstead, Keith Gull
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bernardo J FothDominique Soldati
Apr 4, 2006·Developmental Biology·Frantisek BaluskaPeter W Barlow
Nov 21, 2007·Current Opinion in Microbiology·Tansy C HammartonJeremy C Mottram
Nov 28, 2007·BMC Genomics·Rachel BrenchleyLydia Tabernero
Apr 30, 2008·The Journal of Cell Biology·Christopher L de GraffenriedGraham Warren
Dec 24, 2008·PLoS Neglected Tropical Diseases·Eric M FèvrePascal Lutumba
Aug 27, 2009·PLoS Neglected Tropical Diseases·Peter J Hotez, Aruna Kamath
Oct 7, 2009·Nature Reviews. Microbiology·Mark C Field, Mark Carrington
Oct 22, 2009·Nucleic Acids Research·Martin AslettHaiming Wang
Jul 3, 2010·Molecular and Biochemical Parasitology·Balázs Szöör
Dec 7, 2010·Nature Methods·Hyungwon ChoiAlexey I Nesvizhskii

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Citations

Sep 11, 2019·Annual Review of Microbiology·Richard J WheelerJack D Sunter
Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Nov 13, 2020·Molecular Biology of the Cell·Paul C Campbell, Christopher L de Graffenried
Sep 1, 2019·Trends in Parasitology·Amy N Sinclair, Christopher L de Graffenried
Dec 15, 2020·Trends in Parasitology·Clare HallidayJack D Sunter
Oct 15, 2020·Open Biology·Yasuhiro KurasawaZiyin Li

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