PMID: 230508Dec 1, 1979Paper

Identification of transferrin receptors on the surface of human cultured cells

Proceedings of the National Academy of Sciences of the United States of America
T A HamiltonH H Sussman

Abstract

We have examined the binding of human transferrin to cultured human choriocarcinoma cell lines and to detergent extracts of such cells. The results indicate the presence of a high-affinity saturable binding site (Ka = 4.25 x 10(8) M-1) that is specific for transferrin. This receptor has also been detected on three other human cell lines of different phenotypic origin, including Wil-2 (splenic lymphocytes of B-cell origin), RPMI-2650 (a quasi-diploid nasopharyngeal carcinoma), and WI-38 (embryonic lung fibroblasts). By using anti-human transferrin antiserum to immunoprecipitate the receptor-transferrin complex from detergent extracts of cells containing saturating levels of transferrin followed by sodium dodecyl sulfate/polyacrylamide gel electrophoresis, a single polypeptide of 90,000 daltons has been identified as a subunit of the putative transferrin receptor. The protein shows immunochemical identity and coelectrophoreses in sodium dodecyl sulfate gels with a cell surface glycoprotein subunit, previously identified in placental brush border membrane preparations, on all human cultured cell lines examined. These results suggest that the recent demonstration of transferrin dependence of maximal cell growth in culture [e.g., Hu...Continue Reading

References

Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T A HamiltonH H Sussman
Apr 3, 1979·Biochimica Et Biophysica Acta·J W Larrick, P Cresswell
Feb 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·S E Hutchings, G H Sato
Apr 11, 1977·Biochemical and Biophysical Research Communications·P S RudlandL J de Asua
Feb 1, 1979·Experimental Cell Research·D V YoungS C Hartman
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·K NishikawaG Sato
Sep 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H A Armelin
Jul 27, 1970·Biochemical and Biophysical Research Communications·D R Phillips, M Morrison
Mar 1, 1963·The Journal of Clinical Investigation·J H JANDL, J H KATZ
Nov 1, 1964·Acta Physiologica Scandinavica·C B LAURELL, E MORGAN
Oct 1, 1959·Proceedings of the Society for Experimental Biology and Medicine·R HERTZ

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Citations

Jul 1, 1990·American Journal of Hematology·D KlemowB S Skikne
Jan 1, 1987·Cancer Metastasis Reviews·E D Letendre
Jan 1, 1985·Journal of Cancer Research and Clinical Oncology·B VandewalleJ Lefebvre
Dec 1, 1991·Journal of Bioenergetics and Biomembranes·H LöwI L Sun
Mar 1, 1981·Biological Trace Element Research·E D Weinberg
Jan 1, 1984·The International Journal of Biochemistry·A Hirose-KumagaiN Akamatsu
Jan 1, 1981·International Journal of Nuclear Medicine and Biology·R G Sephton, A W Harris
Jan 1, 1984·Molecular Aspects of Medicine·P Knox
Sep 14, 1990·Biochimica Et Biophysica Acta·M K NairJ D Cook
Apr 29, 1982·Biochimica Et Biophysica Acta·C G Gahmberg, L C Andersson
Jan 1, 1985·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·G J MarkelonisS R Max
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I S Trowbridge, M B Omary
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C A EnnsH H Sussman
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R SutherlandM Greaves
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·I S Trowbridge, F Lopez
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P EkblomR Timpl
Jan 1, 1983·Nutrition and Cancer·E D Weinberg
Jan 1, 1985·The Journal of Cell Biology·G J MarkelonisP Azari
Dec 8, 1989·European Journal of Biochemistry·D RoironC Figarella
Dec 31, 1997·American Journal of Reproductive Immunology : AJRI·S Martínez-RománJ A Vanrell
Jan 1, 1984·Journal of Clinical Pathology·R RossielloG G Giordano
Nov 1, 1982·The Journal of Clinical Investigation·L A BoxerR L Baehner
Jul 1, 1983·The Journal of Experimental Medicine·R S MittlerG Goldstein
Jun 30, 1988·Biochemical and Biophysical Research Communications·M F BourgeadeF Besancon
Jan 1, 1994·American Journal of Obstetrics and Gynecology·D W BianchiA F Flint
Oct 1, 1989·Journal of Neuroscience Research·A Espinosa de los MonterosJ de Vellis
Jan 1, 1986·Critical Reviews in Oncology/hematology·S L SchneiderP M Zeltzer
Feb 28, 2014·The Journal of Membrane Biology·Stephanie Tortorella, Tom C Karagiannis
Mar 12, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Ryo TachiyamaHideaki Fujita

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