Identification of transglutaminase-reactive residues in S100A11.

The Journal of Biological Chemistry
N Robinson, R L Eckert

Abstract

The recent finding that S100A11 is a component of the keratinocyte cornified envelope (CE) (Robinson, N. A., Lapic, S., Welter, J. F., and Eckert, R. L. (1997) J. Biol. Chem. 272, 12035-12046) suggests that S100A11 is a transglutaminase (TG) substrate. In the present study we show that S100A11 forms multimers when cultured keratinocytes are challenged by increased levels of intracellular calcium and that multimer formation is inhibited by the TG inhibitor, cystamine. These S100A11 multimers appear to be incorporated into the CE, as immunoreactive S100A11 is detected in purified envelopes prepared from cultured cells and from foreskin epidermis. To study S100A11 as a transglutaminase substrate, recombinant human S100A11 (rhS100A11) was used in a cell-free cross-linking system. [14C]Putrescine, a primary amine, labels rhS100A11 in a TG-dependent manner. Trypsin digestion of [14C]putrescine-labeled rhS100A11 releases one radiolabeled peptide, Ala98-Lys103. The glutamine residue in this segment, Gln102, is the site of radiolabel incorporation indicating that Gln102 functions as an amine acceptor. The ability of S100A11 to form multimers indicates that it also has a reactive lysine residue that functions as an amine donor. To identi...Continue Reading

References

Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C S GreenbergR H Rice
Feb 1, 1991·The Journal of Investigative Dermatology·R PolakowskaL A Goldsmith
Jan 1, 1991·Journal of Biochemical Toxicology·N A RobinsonJ G Pace
Jan 15, 1990·The Biochemical Journal·R H RiceR Chakravarty
Sep 15, 1989·Biochemical and Biophysical Research Communications·Y AndoR Kannagi
Jan 1, 1980·Annual Review of Biochemistry·J E Folk
Jan 1, 1984·Molecular and Cellular Biochemistry·J A Rothnagel, G E Rogers
Oct 1, 1993·Differentiation; Research in Biological Diversity·N SizemoreE A Rorke
Jan 1, 1994·Annual Review of Biophysics and Biomolecular Structure·M A Swairjo, B A Seaton
Jan 1, 1995·Brain Research Bulletin·D B ZimmerW Song
Jan 12, 1996·The Journal of Biological Chemistry·W S MailliardD D Schlaepfer
Oct 1, 1996·The Biochemical Journal·J SeemannV Gerke
Oct 14, 1996·Biochemical and Biophysical Research Communications·R WickiC W Heizmann
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B G AllenJ A Cox
Jun 1, 1983·The Journal of Investigative Dermatology·L A Goldsmith

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Mar 8, 2005·Biochemical Pharmacology·Thomas M JeitnerArthur J L Cooper
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Diana TetiHarold McNair
Oct 15, 2010·Molecular & Cellular Proteomics : MCP·Massimo CastagnolaIrene Messana
Mar 7, 2001·Antioxidants & Redox Signaling·B KaltschmidtC Kaltschmidt
Dec 10, 1999·Molecular Biology of the Cell·P M Steinert, L N Marekov
Apr 22, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ann-Marie BroomeRichard L Eckert
Jan 24, 2009·Developmental and Comparative Immunology·Matthias MichalekJoachim Grötzinger
Mar 2, 2005·The Journal of Investigative Dermatology·Richard L EckertEllen A Rorke
Feb 14, 2004·The Journal of Investigative Dermatology·Ann-Marie Broome, Richard L Eckert
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Apr 20, 2007·Neuroscience Letters·P J S VigL T Sutton
Aug 21, 2003·The Journal of Biological Chemistry·Michael T SturnioloRichard L Eckert
Nov 13, 1998·The Journal of Biological Chemistry·M F ChampliaudP F Olson
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·R ChenT M Saba
Dec 13, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Denise L CecilRobert Terkeltaub
Mar 22, 2001·Biochemistry·M RuseR L Eckert
Sep 16, 1999·Biochemical and Biophysical Research Communications·H InadaC W Heizmann
Dec 8, 2000·Biochimica Et Biophysica Acta·G E DaveyC W Heizmann

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