Identification of transport pathways for citric acid cycle intermediates in the human colon carcinoma cell line, Caco-2

Biochimica Et Biophysica Acta
Jittima Weerachayaphorn, Ana M Pajor

Abstract

Citric acid cycle intermediates are absorbed from the gastrointestinal tract through carrier-mediated mechanisms, although the transport pathways have not been clearly identified. This study examines the transport of citric acid cycle intermediates in the Caco-2 human colon carcinoma cell line, often used as a model of small intestine. Inulin was used as an extracellular volume marker instead of mannitol since the apparent volume measured with mannitol changed with time. The results show that Caco-2 cells contain at least three distinct transporters, including the Na+-dependent di- and tricarboxylate transporters, NaDC1 and NaCT, and one or more sodium-independent pathways, possibly involving organic anion transporters. Succinate transport is mediated mostly by Na+-dependent pathways, predominantly by NaDC1, but with some contribution by NaCT. RT-PCR and functional characteristics verified the expression of these transporters in Caco-2 cells. In contrast, citrate transport in Caco-2 cells occurs by a combination of Na+-independent pathways, possibly mediated by an organic anion transporter, and Na+-dependent mechanisms. The non-metabolizable dicarboxylate, methylsuccinate, is also transported by a combination of Na+-dependent a...Continue Reading

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Citations

Apr 30, 2010·European Journal of Nutrition·Houda Sabboh-JourdanMichael J Gidley
Nov 7, 2013·Journal of Regenerative Medicine & Tissue Engineering·Leslie C Costello, Renty B Franklin
Apr 27, 2010·International Journal of Pharmaceutics·Jonathan GooleGregory T Knipp
May 19, 2009·BioFactors·Rafael Moreno-SánchezJuan Carlos Gallardo-Pérez
Jun 16, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Osamu KimuraTetsuya Endo
Aug 6, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Subrata SabuiHamid M Said
Dec 26, 2018·Nutrients·Jessica ConnorsJohan Van Limbergen

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