Identification of Trenbolone Metabolites Using Hydrogen Isotope Ratio Mass Spectrometry and Liquid Chromatography/High Accuracy/High Resolution Mass Spectrometry for Doping Control Analysis

Frontiers in Chemistry
Marlen PutzMario Thevis

Abstract

Trenbolone is a synthetic anabolic-androgenic steroid, which has been misused for performance enhancement in sports. The detection of trenbolone doping in routine sports drug testing programs is complex as methods utilizing gas chromatography/mass spectrometry are complicated by unspecific derivatization products and artifacts, and liquid chromatography/mass spectrometry-based assays have shown to allow for comparably high limits-of-detection only. The number of previously reported metabolites in human urine is limited, and most analytical methods rely on targeting epitrenbolone, trenbolone glucuronide, and epitrenbolone glucuronide. In order to probe for the presence of additional trenbolone metabolites and to re-investigate the metabolism, an elimination study was conducted. One single dose of 10 mg of 5-fold deuterated trenbolone was administered to a healthy male volunteer and urine samples were collected for 30 days. For sample processing, published protocols were combined considering unconjugated, glucuronic acid-, sulfo- and alkaline-labile conjugated steroid metabolites. The sample preparation strategy consisted of solid-phase extractions, liquid-liquid extractions, metabolite de-conjugation, HPLC fractionation, and der...Continue Reading

References

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Citations

Nov 14, 2020·Drug Testing and Analysis·Mario ThevisHans Geyer
Aug 15, 2021·Journal of Pharmaceutical and Biomedical Analysis·Mario ThevisAndreas Thomas
Aug 20, 2021·Journal of Analytical Toxicology·Laurie GheddarPascal Kintz

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Methods Mentioned

BETA
Acetylation
nuclear magnetic resonance

Software Mentioned

MassHunter
Xcalibur
Isodat

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