PMID: 8098897Apr 1, 1993Paper

Identification of Trypanosoma brucei gambiense by PCR amplification of variant surface glycoprotein genes

Acta Tropica
T J BromidgeP Dukes

Abstract

We have developed a sensitive and specific method to identify Trypanosoma brucei gambiense using the polymerase chain reaction (PCR) to amplify the gene encoding variant surface glycoprotein (VSG) Antat 11.17. The test was capable of distinguishing T. b. gambiense from T. b. brucei in most foci of gambian sleeping sickness and gave positive results with previously well-characterised Type I T. b. gambiense stocks from Ivory Coast, Nigeria, Cameroon, Congo, Zaire and Sudan. The test gave negative results with T. b. rhodesiense from Zambia, Kenya and Uganda, virulent or Type II T. b. gambiense from Ivory Coast and T. b. brucei stocks from East and West Africa. The test was modified for colorimetric detection in dot blot format by using nested biotinylated primers in a two-step reaction. Comparison of DNA sequences of VSG genes from T. b. gambiense and other T. brucei ssp. stocks showed a high level of homology, suggesting recent gene flow.

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Citations

Jun 1, 1995·Molecular and Biochemical Parasitology·F Mathieu-DaudéM McClelland
Feb 1, 1997·Acta Tropica·G D KanmogneW C Gibson
Jul 15, 1999·International Journal for Parasitology·J R StevensW C Gibson
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Wendy Gibson
Apr 26, 2000·Molecular and Biochemical Parasitology·W GibsonJ Stevens
Feb 29, 2000·Molecular and Biochemical Parasitology·N BiteauT Baltz
Dec 11, 2002·Trends in Parasitology·Wendy Gibson
Nov 26, 2003·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Imna MaleleWendy Gibson
Feb 11, 2011·Journal of Clinical Microbiology·Z K NjiruE Matovu
Dec 10, 2003·Journal of Clinical Microbiology·Noritaka KubokiIkuo Igarashi
Dec 15, 2010·Diagnostic Microbiology and Infectious Disease·Frank L BasiyeHenk D F H Schallig
Sep 16, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Liam J MorrisonAnnette MacLeod
Mar 23, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Wendy GibsonJoseph Ndung'u
Jun 1, 2006·Advances in Parasitology·E M FèvreI Maudlin
Jan 14, 2000·The Korean Journal of Parasitology·P A MbatiH Hirumi
Aug 29, 2019·Parasites & Vectors·Paschal Ugochukwu UmeakuanaBoniface Maduka Anene
Nov 2, 2019·Trends in Parasitology·Vincent JamonneauThierry De Meeus

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