Identification of tumour-induced changes in endothelial cell surface protein expression: an in vitro model

The International Journal of Biochemistry & Cell Biology
P Hewett

Abstract

The selective destruction of the supporting vasculature of tumours has been proposed as a means of therapy. Fundamental to this approach is the identification of suitable targets on tumour-endothelium. To detect proteins that may be up-regulated on the luminal (apical) surface of tumour-associated endothelium confluent endothelial cells were examined following incubation with tumour cell conditioned medium (TCM) from, or co-culture with, a range of breast carcinoma and small cell lung carcinoma (SCLC) cell lines. Exposed endothelial membrane proteins were labelled with sulpho-NHS-biotin and detected by enhanced chemiluminescence following two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and western blotting. TCM induced varying levels of proliferative activity in endothelial cells; generally breast TCM contained greater mitogenic activity than SCLC TCM. Exposure of human breast and lung microvascular, and umbilical vein endothelial cells to soluble tumour cell factors from several breast cancer and SCLC cells lines produced similar changes in luminal protein profiles: Breast cancer cells and in particular the MDA-MB-231 cell line induced the most pronounced changes. The expression of six proteins was altered consis...Continue Reading

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Citations

Jun 10, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Shuhui LiangDaiming Fan
Feb 2, 2016·Biochimica Et Biophysica Acta·Atsushi NakanoNathan J Palpant
Apr 19, 2007·The Prostate·Hayley C WhitakerDavid E Neal
Feb 26, 2015·Human Vaccines & Immunotherapeutics·Petr G Lokhov, Elena E Balashova
Oct 24, 2003·Molecular & Cellular Proteomics : MCP·Matthew J PeirceAndrew P Cope
Aug 13, 2011·American Journal of Physiology. Cell Physiology·Alessandra Fiorio PlaIndu S Ambudkar
Oct 3, 2012·International Journal of Molecular Medicine·Shuangsheng HuangXiaosu Zhang
Aug 26, 2003·British Journal of Nursing : BJN·Melanie Ottewill
Dec 21, 2002·The Journal of Biological Chemistry·Bong Kyung ShinSamir M Hanash
Aug 14, 2002·World Journal of Gastroenterology : WJG·Juan RenBo-Rong Pan

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