Identification of tumour initiating cells in feline head and neck squamous cell carcinoma and evidence for gefitinib induced epithelial to mesenchymal transition

The Veterinary Journal
L Y PangD J Argyle

Abstract

Feline oral squamous cell carcinoma is considered a highly invasive cancer that carries a high level of morbidity. Despite aggressive surgery, patients often succumb to disease, the tumour having inherent insensitivity to radiation and chemotherapy. In this study we sought to identify cells within the feline SCC1 line that have stem cell properties, including inherent resistance mechanisms. When feline cells were subjected to harsh growth conditions, they formed sphere colonies consistent with a stem cell phenotype. Utilising CD133, we were able to identify a small fraction of cells within the population that had enhanced sphere-forming ability, reduced sensitivity to radiation and conventional chemotherapy and demonstrated resistance to the EGFR-targeting drug, gefitinib. In addition, long-term culture of feline SSC1 cells in gefitinib caused a change in cell morphology and gene expression reminiscent of an epithelial to mesenchymal transition. Taken together, these results suggest that feline SCC may be driven by small subset of cancer stem cells.

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Citations

Aug 24, 2013·Pathology Research International·Jackie M Wypij
Aug 7, 2012·Seminars in Cancer Biology·Philip L LeopoldHongjun Wang
Mar 12, 2016·The Veterinary Journal·Wachiraphan SupsavhadThomas J Rosol
Jan 31, 2015·The Veterinary Journal·Lisa Y Pang, David J Argyle
Apr 14, 2012·The Veterinary Journal·Laura Snyder
Sep 18, 2013·Veterinary Immunology and Immunopathology·Masaki MichishitaKimimasa Takahashi
May 4, 2016·Clinical & Experimental Metastasis·YeonHee MoonWonbong Lim
Aug 7, 2013·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Mian XieLi Zhang
Nov 28, 2017·Endocrine-related Cancer·Y CapodannoD J Argyle
Jun 30, 2015·Veterinary Sciences·Claire M Cannon
Jul 23, 2015·Journal of Veterinary Dentistry·Ozgur BilgicJohn R Lewis

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