Identification of Tup1 and Cyc8 mutations defective in the responses to osmotic stress

Biochemical and Biophysical Research Communications
Yoshifumi KobayashiTokichi Miyakawa

Abstract

In the yeast Saccharomyces cerevisiae, Tup1, in association with Cyc8 (Ssn6), functions as a general transcriptional corepressor. This repression is mediated by recruitment of the Tup1-Cyc8 complex to target promoters through sequence-specific DNA-binding proteins such as Sko1, which mediates the HOG pathway-dependent regulation. We identified tup1 and cyc8 mutant alleles as the suppressor of osmo-sensitivity of the hog1Delta strain. In these mutants, although the expression of the genes under the control of DNA-binding proteins other than Sko1 was apparently normal, the Sko1-regulated genes GRE2 and AHP1 were derepressed under non-stress conditions, suggesting that the Tup1 and Cyc8 mutant proteins were specifically defective in the repression of the Sko1-dependent genes. Chromatin immunoprecipitation analyses of the GRE2 promoter in the mutants demonstrated that the Sko1-Tup1-Cyc8 complex was localized to the promoter, together with Gcn5/SAGA, suggesting that the erroneous recruitment of SAGA to the promoter led to the derepression.

References

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Citations

Sep 8, 2012·Molecular Biology of the Cell·Clàudia Ruiz-RoigEulàlia de Nadal
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Nov 28, 2009·The EMBO Journal·Eulàlia de Nadal, Francesc Posas
Sep 30, 2014·Current Genetics·Carme SoléFrancesc Posas
Jan 25, 2019·Bioscience, Biotechnology, and Biochemistry·Koji MasumuraMasaki Mizunuma

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