Identification of two additives, locust bean gum (E-410) and guar gum (E-412), in food products by DNA-based methods

Food Additives and Contaminants
Mercedes UrdiaínJosep A Rosselló

Abstract

Locust bean gum (E-410) and guar gum (E-412) are high molecular weight galactomannans used by the food industry as versatile food additives. The compounds, although chemically closely related, do not have the same functional properties when used in foods, and the substitution or unadvertised addition of either could change the desired qualities of the product. Analytical discrimination between E-410 and E-412 is technically difficult since they only differ in their galactose: mannose ratios, being 1 : 4 and 1 : 2 for locust bean gum and guar gum, respectively. A qualitative DNA-based method is reported for the authentication of additives E-410 and E-412 in finished food products (ice cream, dehydrated desserts, milk derivatives, dehydrated soups, salad dressing, marmalade and meat) from small quantities of food. DNA sequences from the nuclear ribosomal spacers of Ceratonia siliqua and Cyamopsis tetragonoloba, the plant sources of E-410 and E-412, respectively, were used to design polymerase chain reaction primers specific for each additive (PA23/PA21 and PG22/PG21). Twenty-two foods were analysed for the presence of E-410 and E-412 additives by this single-step polymerase chain reaction-based method. Positive DNA amplifications...Continue Reading

References

Nov 1, 1991·Plant Molecular Biology·P TaberletJ Bouvet
Nov 1, 1996·Organizational Behavior and Human Decision Processes·R Dusenbury, M G Fennema
Mar 21, 2002·Journal of Agricultural and Food Chemistry·Christophe CordellaLouisette Lizzani-Cuvelier

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Citations

Feb 20, 2014·International Journal of Biological Macromolecules·Sheweta Barak, Deepak Mudgil
Aug 28, 2012·Journal of Pharmacy & Bioallied Sciences·Marita Dionísio, Ana Grenha
Feb 24, 2017·EFSA Journal·UNKNOWN EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS)Birgit Dusemund
Mar 18, 2021·PloS One·Tatiane Fernanda LeonelEliana Gertrudes de Macedo Lemos
Apr 28, 2005·Journal of Agricultural and Food Chemistry·Mercedes UrdiaínJosep A Rosselló

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Methods Mentioned

BETA
electrophoresis
light microscopy
PCR
restriction profiles
electrophoretic

Software Mentioned

BLAST
OLIGO

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