PMID: 8590318Feb 1, 1995Paper

Identification of two forms (31-33 and 48 kD) of the urinary soluble p55 tumor necrosis factor receptor that are differentially N- and O-glycosylated

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
A CortiG Cassani

Abstract

The structure and the activity of urinary soluble TNF receptor type 1 (sTNF-R1), isolated from the urine of normal individuals, has been characterized and compared with that of recombinant sTNF-R1 expressed in CHO cells and with that of a nonglycosylated form expressed in Escherichia coli. Urinary sTNF-R1 was resolved in a major band of 31-33 kD and in a 48 kD band (less than 5% of total) by reducing SDS-PAGE; CHO sTNF-R1 was resolved in two bands of 29 and 31 kD. All bands were recognized by various anti-sTNF-R1 antibodies as well as by TNF-alpha in western and ligand blotting assays. No cross-reaction was observed with anti-TNF-R2 antibodies. N- and O-glycosylation studies indicated that (1) the 29-31 kD recombinant form as well as the 31-33 kD urinary form are N-glycosylated; (2) the differences between the 29-31 and 31-33 kD recombinant and natural products are mainly related to differences in the N-linked sugar content; and (3) the 48 kD sTNF-R1 isolated from urine also contains O-linked sugars. The urinary sTNF-R1 antigen mixture was able to inhibit TNF-alpha cytotoxicity with a potency comparable to that of nonglycosylated E. coli sTNF-R1. At variance, urinary sTNF-R1 was able to inhibit TNF-beta sevenfold more efficient...Continue Reading

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Citations

May 27, 1996·Journal of Immunological Methods·A MeagerA Mire-Sluis
Oct 22, 2003·Pathophysiology : the Official Journal of the International Society for Pathophysiology·C P. CoyneBrad W. Fenwick
Sep 21, 2011·The Journal of Biological Chemistry·Zhongyu LiuSusan L Bellis
Jul 6, 2005·Journal of Inflammation·Christoph ScherüblDaniela N Männel
Aug 31, 2007·Biologicals : Journal of the International Association of Biological Standardization·Rodney KeckAndrew Jones
Mar 3, 2018·International Journal of Molecular Sciences·Olivier Micheau
Oct 30, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Georg H WaetzigStefan Schreiber
Dec 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M TerlizzeseF Antonetti
Jul 6, 2006·Journal of Agricultural and Food Chemistry·Satomi YanoHirofumi Tachibana

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