Identification of two juvenile hormone inducible transcription factors from the silkworm, Bombyx mori

Journal of Insect Physiology
Hitoshi MatsumotoM Kamimura

Abstract

Juvenile hormone (JH) regulates many physiological processes in insects. However, the signal cascades in which JH is active have not yet been fully elucidated, particularly in comparison to another major hormone ecdysteroid. Here we identified two JH inducible transcription factors as candidate components of JH signaling pathways in the silkworm, Bombyx mori. DNA microarray analysis showed that expression of two transcription factor genes, E75 and Enhancer of split mβ (E(spl)mβ), was induced by juvenile hormone I (JH I) in NIAS-Bm-aff3 cells. Real time RT-PCR analysis confirmed that expression of four E75 isoforms (E75A, E75B, E75C and E75D) and E(spl)mβ was 3-8 times greater after JH I addition. Addition of the protein synthesis inhibitor cycloheximide did not suppress JH-induced expression of the genes, indicating that they were directly induced by JH. JH-induced expression of E75 and E(spl)mβ was also observed in four other B. mori cell lines and in larval hemocytes of final instar larvae. Notably, E75A expression was induced very strongly in larval hemocytes by topical application of the JH analog fenoxycarb; the level of induced expression was comparable to that produced by feeding larvae with 20-hydroxyecdysone. These res...Continue Reading

References

Jun 1, 1995·Insect Biochemistry and Molecular Biology·S R PalliA Retnakaran
Jan 28, 1998·Annual Review of Entomology·T S DhadiallaD P Le
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kazuei MitaSusumu Maeda
May 23, 2007·Insect Biochemistry and Molecular Biology·Robert B BecksteadCarl S Thummel
Nov 27, 2007·Mechanisms of Development·Chieka MinakuchiLynn M Riddiford
Feb 25, 2009·Genome Research·Zareen GauharKevin P White
Jun 26, 2009·Insect Biochemistry and Molecular Biology·Kiyoshi Hiruma, Lynn M Riddiford
Oct 22, 2009·BMC Genomics·Michihiko ShimomuraKazuei Mita
Dec 19, 2009·Insect Biochemistry and Molecular Biology·Jinsong ZhuXing Zhang
Sep 11, 2010·The Journal of Biological Chemistry·Yasushi KanamoriManabu Kamimura
Nov 3, 2011·Insect Biochemistry and Molecular Biology·Laetitia BigotDavid Siaussat
Mar 20, 2012·The Journal of Biological Chemistry·Manabu KamimuraMakoto Kiuchi
Sep 22, 2012·Annual Review of Entomology·Marek JindraLynn M Riddiford
Sep 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sang Woon ShinAlexander S Raikhel
May 8, 2013·Bioscience, Biotechnology, and Biochemistry·Kenjiro FurutaTakahiro Shiotsuki
Dec 1, 1996·Development Genes and Evolution·M Jindra, L M Riddiford
Jun 17, 2014·Insect Biochemistry and Molecular Biology·Yingjun CuiSubba Reddy Palli
Jul 16, 2014·Insect Biochemistry and Molecular Biology·Jiasheng SongShutang Zhou

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