Identification of two new genes, mukE and mukF, involved in chromosome partitioning in Escherichia coli

Molecular & General Genetics : MGG
K YamanakaS Hiraga

Abstract

We have previously reported that the MukB protein is essential for chromosome partitioning in Escherichia coli and that mukB mutants produce anucleate cells and are temperature-sensitive for colony formation. The mukB gene maps at 21 min on the E. coli chromosome and smtA-mukF-mukE-mukB genes might comprise an operon, which is transcribed in a clockwise direction. Here, we report that mukF and mukE null mutants are both temperature-sensitive for colony formation and produce anucleate cells even at the permissive temperature. These phenotypes are the same as those observed in the mukB null mutant. The primary sequence of MukF includes a leucine zipper structure and an acidic domain. Mutational analysis revealed that both are required for MukF function. When the MukF protein was overproduced in the wild-type strain, anucleate cells were produced. In contrast, overproduction of either MukE or MukB did not cause the defect. In null mutants for the mukF, mukE, and mukB genes, the synchronous initiation of chromosome replication was not affected. The mini-F plasmid was as stably maintained in these mutants as in the wild-type strain. These results indicate that the MukF, MukE, and MukB proteins are involved in the chromosome partitio...Continue Reading

References

Jan 1, 1992·Annual Review of Biochemistry·S Hiraga
Aug 1, 1992·The Biochemical Journal·M MichalakM Opas
Oct 1, 1991·Journal of Bacteriology·B EzakiS Hiraga
Feb 1, 1991·Research in Microbiology·S HiragaA Jaffé
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1987·The Journal of Cell Biology·W C EarnshawD W Cleveland
Dec 1, 1988·Genes & Development·F J RauscherT Curran
Dec 15, 1988·Nature·T Kouzarides, E Ziff
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Ogura, S Hiraga
Jul 28, 1995·Science·R D FleischmannJ M Merrick
May 1, 1995·Journal of Bacteriology·B E Funnell, L Gagnier
Jan 1, 1993·Annual Review of Microbiology·W D Donachie
Oct 1, 1993·Current Opinion in Genetics & Development·S Hiraga
Sep 1, 1993·Journal of Bacteriology·K Skarstad, E Boye

❮ Previous
Next ❯

Citations

Sep 1, 2005·Molecular Genetics and Genomics : MGG·Shun AdachiSota Hiraga
Mar 25, 2009·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Peter L Graumann, Tobias Knust
Mar 31, 1999·Trends in Genetics : TIG·M E Sharpe, J Errington
Jul 6, 2000·Trends in Microbiology·J Møller-JensenK Gerdes
Jul 24, 1998·Trends in Microbiology·W Margolin
Mar 12, 2002·Molecular Microbiology·Y SunakoS Hiraga
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ryo Hayama, Kenneth J Marians
Oct 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yinyin LiMartha G Oakley
Feb 5, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Masatoshi Esaki, Teru Ogura
Nov 13, 2004·Genome Biology·Brian J PeterNicholas R Cozzarelli
Jan 1, 2014·PloS One·Andrea L EdwardsValentin V Rybenkov
Feb 8, 2000·Proceedings of the National Academy of Sciences of the United States of America·J A Sawitzke, S Austin
Nov 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Elena A KouzminovaAndrei Kuzminov
Oct 15, 2013·FEMS Microbiology Reviews·Sophie Nolivos, David Sherratt
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael W PanasWilliam R Jacobs
Jul 25, 2006·Journal of Structural Biology·Martin Thanbichler, Lucy Shapiro
Apr 7, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christine D HardyNicholas R Cozzarelli
Aug 14, 1998·Molecular Microbiology·J P Bouché, S Pichoff
Sep 20, 2006·Journal of Molecular Microbiology and Biotechnology·Ronen HazanBen-Yehuda Sigal
Jul 15, 2011·Molecular Microbiology·Zoya M PetrushenkoValentin V Rybenkov
Sep 20, 2011·Critical Reviews in Biochemistry and Molecular Biology·Sidney D Carter, Camilla Sjögren
Dec 25, 2016·The EMBO Journal·Rodrigo Arias-CartinChristine Jacobs-Wagner
Jan 25, 2018·FEMS Microbiology Reviews·Liselot DewachterJan Michiels
Jul 4, 2012·Journal of Bacteriology·Anjana BadrinarayananDavid Sherratt
Dec 27, 2011·The Journal of Biological Chemistry·Rebekah FramptonGregory M Cook
Aug 27, 2017·The Journal of Biological Chemistry·Rupesh KumarKenneth J Marians
Oct 5, 2016·The Journal of Biological Chemistry·Soon BahngKenneth J Marians
Aug 1, 2009·EcoSal Plus·Akira Ishihama
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.