Identification of two nuclear factor of activated T-cells (NFAT)-response elements in the 5'-upstream regulatory region of the ET-1 promoter.

The Journal of Biological Chemistry
Kevin A StraitDonald E Kohan

Abstract

Collecting duct-derived ET-1 regulates salt excretion and blood pressure. We have reported the presence of an inner medullary collecting duct (IMCD)-specific enhancer region in the 5'-upstream ET-1 promoter (Strait, K. A., Stricklett, P. K., Kohan, J. L., Miller, M. B., and Kohan, D. E. (2007) Am. J. Physiol. Renal Physiol. 293, F601-F606). The current studies provide further characterization of the ET-1 5'-upstream distal promoter to identify the IMCD-specific enhancer elements. Deletion studies identified two regions of the 5'-upstream ET-1 promoter, -1725 to -1319 bp and -1319 to -1026 bp, which were required for maximal promoter activity in transfected rat IMCD cells. Transcription factor binding site analysis of these regions identified two consensus nuclear factor of activated T-cells (NFAT) binding sites at -1263 and -1563. EMSA analysis using nuclear extracts from IMCD cells showed that both the -1263 and the -1563 NFAT sites in the ET-1 distal promoter competed for NFAT binding to previously identified NFAT sites in the IL-2 and TNF genes. Gel supershift analysis showed that each of the NFAT binding sites in the ET-1 promoter bound NFAT proteins derived from IMCD nuclear extracts, but they selectively bound different N...Continue Reading

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Citations

Jan 19, 2016·Life Sciences·Amanda K WelchCharles S Wingo
Feb 8, 2011·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Fernando Rodríguez-PascualSantiago Lamas
Jan 10, 2012·British Journal of Pharmacology·A K WelchB D Cain
Nov 8, 2015·Life Sciences·Meghana M PanditDonald E Kohan
Aug 30, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Donald E Kohan
Jan 16, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ali Mohammad Pourbagher-ShahriSaeed Samargahndian

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