Identification of two phylogenetically related organisms from feces by PCR for detection of Salmonella spp.

Journal of Clinical Microbiology
C Gentry-WeeksMichael Jessen

Abstract

Two previously reported PCR methods were evaluated to determine whether they are as sensitive and specific as conventional culture methods in detecting Salmonella spp. from feces. Bovine and equine feces were enriched overnight in brain heart infusion broth and assayed using PCR methods and primer sets described by other investigators. A total of 774 fecal specimens were tested using a primer set (invE-A primer set) that amplifies a region spanning the invasin E and A genes of Salmonella enterica serovar Typhimurium. A subset of these fecal specimens (306 of the 774 total) were tested using primers (hisJ primer set) that amplify a portion of the histidine transport J gene. The PCR required 24 h to obtain results, whereas it took 5 to 7 days to identify Salmonella spp. by culture. PCR detection of Salmonella spp. using the hisJ primers and the invE-A primers had a sensitivity of 93.3 and 80%, respectively, and a specificity of 85.6 and 98.6%, respectively, compared with bacterial culture. Amplification of 42 culture-negative fecal specimens (of 306 total specimens) generated a DNA fragment that corresponded to the molecular weight of the amplified hisJ gene. The hisJ-generated amplicons from six culture-negative and six culture-...Continue Reading

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Citations

Dec 17, 2009·Foodborne Pathogens and Disease·Kamelia M OsmanMoustafa I Radwan
May 5, 2010·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Marguerite WouafoAntoinette Ngandjio
Sep 13, 2002·American Journal of Veterinary Research·P Brett KurowskiClaudia R Gentry-Weeks
Dec 11, 2003·Journal of the American Veterinary Medical Association·Catherine A AlinoviChing Ching Wu
Apr 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Michael P WardChing Ching Wu
Aug 12, 2003·Journal of Medical Microbiology·S G PathmanathanK L Thong
Dec 3, 2004·Applied and Environmental Microbiology·Burkhard MalornyReiner Helmuth

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