PMID: 7541640May 1, 1995Paper

Identification of two transcripts of AML1/ETO-fused gene in t(8;21) leukemic cells and expression of wild-type ETO gene in hematopoietic cells

Genes, Chromosomes & Cancer
T EraK Takatsuki

Abstract

The t(8;21) is a common chromosomal abnormality, preferentially associated with acute leukemia showing features of myeloid differentiation. Recently, two genes--AML1, which has a unique runt domain, and ETO (MTG8)--have been isolated from the chromosomal breakpoint. In this study, we isolated and identified two fused transcripts from a leukemic cell line carrying t(8;21). AML1 and ETO were fused at the same position in these transcripts. One of the transcripts codes a unique domain, including two zinc finger domains and three proline- and one leucine-rich region. The other transcript codes only for one proline- and leucine-rich region but lacks zinc finger domains. We demonstrated by polymerase chain reaction (PCR) analysis that 1) these two transcripts are consistently expressed in leukemic cells with t(8;21) obtained from patients and 2) expression of AML1 was not restricted to the particular stage of hematopoietic differentiation but was present in all hematopoietic cells investigated. We also provide evidence that two wild types of ETO transcripts containing the region of the ETO gene in fused transcripts are expressed in hematopoietic cells from different lineages. The widespread expression of AML1 and ETO in hematopoietic...Continue Reading

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Citations

Nov 28, 2001·International Journal of Hematology·R B Lorsbach, J R Downing
Jul 14, 1998·Gene·J K Wolford, M Prochazka
Dec 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Ming YanDong-Er Zhang
Jun 26, 2012·The Journal of Biological Chemistry·Michinori KitagawaTakumi Era
Mar 23, 2011·Biochemistry. Biokhimii︠a︡·E V Chetverina, A B Chetverin
Jan 29, 2013·Molekuliarnaia biologiia·E N MarkovaO L Kantidze
Aug 28, 1998·Leukemia & Lymphoma·N AsouK Takatsuki
Sep 1, 2015·The International Journal of Biochemistry & Cell Biology·Vasily V GrinevOlga V Aleinikova
Jan 29, 2005·Experimental Hematology·Sofia Rondin LindbergInge Olsson
Dec 6, 2000·Seminars in Cell & Developmental Biology·J R DowningA E Yeoh
Aug 14, 2019·Wiley Interdisciplinary Reviews. RNA·Carla NecklesNatasha J Caplen
Jun 21, 2003·Clinical Chemistry·Run Zhang ShiJanet D Rowley
Jan 14, 1999·Genes, Chromosomes & Cancer·S TaviauxM Lafage-Pochitaloff

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