Identification of type-specific and cross-reactive neutralizing conformational epitopes on the major capsid protein of human papillomavirus type 31

Archives of Virology
Maxime J J FleuryPierre Coursaget

Abstract

The majority of the neutralizing epitopes of papillomaviruses (PV) are conformation-specific and have not been fully characterised. Studies have, to date, been limited to a few HPV types only. We analysed the epitopes on the major capsid protein (L1) of Human papillomavirus (HPV) type 31 using monoclonal antibodies (MAbs) generated against HPV-31 virus-like particles (VLPs). The type-specific MAbs against HPV-31 were all found to be neutralizing and recognized conformation-dependent epitopes. Two other MAbs directed against a conformational epitope were found to be cross-reactive with other HPV types, and one of them was found to be cross-neutralizing. Cross-reactive antibodies were further investigated using wild-type HPV-16 L1 VLPs and two mutants. The results obtained suggested the existence of a cross-neutralizing conformational epitope at the N-terminal part of the FG loop of the major capsid protein, and the other four cross-reactive MAbs recognized epitopes also located at the N-terminal part of the FG loop.

References

Jul 1, 1992·The Journal of General Virology·J F VautherotJ Laporte
Nov 1, 1990·Journal of Virology·N D ChristensenP A Welsh
Nov 1, 1994·Journal of Virology·R B RodenJ T Schiller
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·J A SuzichR Schlegel
Aug 19, 1999·The Journal of Pathology·J M WalboomersN Muñoz
Feb 13, 2001·Vaccine·T GiroglouR C Rose
Oct 17, 2001·The Journal of General Virology·Katharina SlupetzkyReinhard Kirnbauer
Feb 22, 2002·International Journal of Cancer. Journal International Du Cancer·Alba-Lucia CombitaPierre Coursaget
Nov 22, 2002·The New England Journal of Medicine·Laura A KoutskyUNKNOWN Proof of Principle Study Investigators
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
May 29, 2003·The Journal of General Virology·Xin-Min WangWilliam L McClements

❮ Previous
Next ❯

Citations

Jun 28, 2007·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A González Martín
Apr 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hannah H AlphsRichard B S Roden
Feb 26, 2010·Journal of Clinical Microbiology·Antoine TouzéPierre Coursaget
Mar 26, 2010·Journal of Translational Medicine·Nicolas CombelasAntoine Touzé
Dec 21, 2010·Annual Review of Immunology·Ian H FrazerStephen R Mattarollo
Oct 27, 2009·Archives of Medical Research·Vicente Madrid-MarinaAlejandro García-Carrancá
Sep 22, 2009·La Presse médicale·Christiane MouginPierre Coursaget
Nov 1, 2015·The Journal of Infectious Diseases·Ariana HarariUNKNOWN Costa Rica HPV Vaccine Trial Group
Jun 18, 2009·Protein Science : a Publication of the Protein Society·Maxime J J FleuryPierre Coursaget
Feb 3, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ana Pastora Otero-MottaMaría Del Mar Abad
Sep 5, 2006·Vaccine·Margaret StanleyIan Frazer
Mar 10, 2015·Human Vaccines & Immunotherapeutics·Xiao ZhangQinjian Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds