Identification of Tyr-762 in the platelet-derived growth factor alpha-receptor as the binding site for Crk proteins

Oncogene
K YokoteS Mori

Abstract

Tyr-762 is an autophosphorylation site in the human platelet-derived growth factor (PDGF) alpha-receptor. In order to investigate whether phosphorylated Tyr-762 serves as a docking site for downstream signal transduction molecules, affinity purification using an immobilized synthetic peptide containing phosphorylated Tyr-762 and its surrounding amino acid residues was performed. Proteins in HeLa cell lysate of molecular sizes 27, 38 and 40 kDa bound to the phosphorylated, but not to the unphosphorylated peptide. Analyses of partial amino acid sequences of the purified proteins indicated that they were identical to CrkI, CrkII and CrkL respectively. The wild-type PDGF alpha-receptor, when expressed in porcine aortic endothelial cells, formed complexes with CrkII and CrkL upon ligand stimulation, which was specifically inhibited by a synthetic peptide containing phosphorylated Tyr-762. Replacement of Tyr-762 with a phenylalanine residue in the PDGF alpha-receptor abrogated ligand-induced binding of Crk proteins. Tyrosine phosphorylation of CrkII and CrkL increased by 1.8- and 1.3-fold, respectively, upon ligand stimulation of the wild-type alpha-receptor. In contrast, the Y762F mutant PDGF alpha-receptor failed to induce tyrosine...Continue Reading

Citations

Feb 25, 2006·Blood·Valerie L GossRoberto D Polakiewicz
May 12, 2009·Cell Communication and Signaling : CCS·Raymond B BirgeShinya Tanaka
Dec 22, 1999·Biochemical and Biophysical Research Communications·T ImaizumiK Yamamura
Jun 8, 2001·Biochemical and Biophysical Research Communications·D ParkY Yun
May 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Melanie Van StryKaren Symes
Apr 30, 2011·Chinese Journal of Cancer·Jheri DupartJonathan C Trent
Jan 15, 2013·Trends in Biochemical Sciences·Lauren E Locascio, Daniel J Donoghue
Nov 9, 2015·Cytokine & Growth Factor Reviews·Erawan Borkham-Kamphorst, Ralf Weiskirchen
Apr 22, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Motonori TakahashiYoshimi Takai
Jun 23, 2004·Cytokine & Growth Factor Reviews·Michelle Tallquist, Andrius Kazlauskas
Jan 25, 2003·Journal of Biochemistry and Molecular Biology·Jiuhong YuHyeong-Reh Choi Kim
Aug 30, 2011·European Journal of Cell Biology·Claudia R C van RoeyenJürgen Floege
Mar 4, 2011·Bioscience Reports·Vegesna RadhaKotagiri Sasikumar
Jul 13, 2004·Biochimica Et Biophysica Acta·David Chodniewicz, Richard L Klemke
Oct 3, 1999·Physiological Reviews·C H Heldin, B Westermark
Sep 11, 2007·Molecular and Cellular Biochemistry·Denise SmithBruce E Bejcek
Jul 25, 2017·The American Journal of Pathology·Alexander KikuchiSatdarshan P Monga
Sep 18, 1998·Biochimica Et Biophysica Acta·C H HeldinL Rönnstrand

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.