Identification of Uncultured Bacterial Species from Firmicutes, Bacteroidetes and CANDIDATUS Saccharibacteria as Candidate Cellulose Utilizers from the Rumen of Beef Cows

Microorganisms
Lee James OpdahlBenoit St-Pierre

Abstract

The ability of ruminants to utilize cellulosic biomass is a result of the metabolic activities of symbiotic microbial communities that reside in the rumen. To gain further insight into this complex microbial ecosystem, a selection-based batch culturing approach was used to identify candidate cellulose-utilizing bacterial consortia. Prior to culturing with cellulose, rumen contents sampled from three beef cows maintained on a forage diet shared 252 Operational Taxonomic Units (OTUs), accounting for 41.6-50.0% of bacterial 16S rRNA gene sequences in their respective samples. Despite this high level of overlap, only one OTU was enriched in cellulose-supplemented cultures from all rumen samples. Otherwise, each set of replicate cellulose supplemented cultures originating from a sampled rumen environment was found to have a distinct bacterial composition. Two of the seven most enriched OTUs were closely matched to well-established rumen cellulose utilizers (RuminococcusflavefaciensandFibrobactersuccinogenes), while the others did not show high nucleotide sequence identity to currently defined bacterial species. The latter were affiliated toPrevotella(1 OTU), Ruminococcaceae (3 OTUs), and the candidate phylum Saccharibacteria (1 OTU)...Continue Reading

References

May 1, 1992·Molecular & General Genetics : MGG·P E Vercoe, K Gregg
Jul 1, 1990·Molecular & General Genetics : MGG·W Y Wang, J A Thomson
Jun 1, 1990·Applied and Environmental Microbiology·K C Doerner, B A White
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Aug 1, 1993·The Proceedings of the Nutrition Society·G T Macfarlane, S Macfarlane
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 26, 2003·FEMS Microbiology Reviews·Denis O KrauseChristopher S McSweeney
May 22, 2004·BioTechniques·Zhongtang Yu, Mark Morrison
Apr 6, 2006·International Journal of Systematic and Evolutionary Microbiology·Defeng XingLihua Zhao
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Jan 9, 2008·Nature Reviews. Microbiology·Harry J FlintBryan A White
Dec 25, 2008·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Edward A BayerHarry J Flint
Jan 6, 2009·FEMS Microbiology Ecology·James B RussellPaul J Weimer
Feb 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jennifer M BrulcBryan A White
Feb 2, 2010·Applied and Environmental Microbiology·S KoikeY Kobayashi
Aug 18, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Philip K Thornton
Jan 13, 2011·FEMS Microbiology Ecology·Minseok KimZhongtang Yu
Jan 29, 2011·Science·Matthias HessEdward M Rubin
May 1, 2008·Animal : an International Journal of Animal Bioscience·R John Wallace
May 11, 2012·Gut Microbes·Harry J FlintEvelyne Forano
May 18, 2012·The ISME Journal·David R JohnsonMartin Ackermann
Feb 22, 2013·The ISME Journal·Elie JamiItzhak Mizrahi
Nov 19, 2013·Nucleic Acids Research·Aidan C Parte
Jul 6, 2014·Microbial Biotechnology·Christopher J CreeveySinead C Leahy
Nov 7, 2015·FEMS Microbiology Ecology·Sharon A HuwsAlison H Kingston-Smith

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