PMID: 3747514Jul 1, 1986Paper

Identification of uterine nuclear type II estrogen binding sites in estrogen treated rats

Journal of Steroid Biochemistry
D M SheehanC R Lyttle

Abstract

Uterine nuclear fractions from estrogen-treated rats contain both the estrogen receptor and a lower affinity estrogen binding site (type II site). In Scatchard plots of estrogen binding, two types of curves are seen. The hook-shaped form is composed of a linear component (the estrogen receptor) and a convex component (the type II site) while the curvilinear form is resolvable into two linear binding species (the estrogen receptor and a secondary site). To clarify the relationship between the two forms, we examined the curvilinear form from immature rats injected for 4 days with estradiol (E2) for type II site properties. Like the hook-shaped type II, this form could be detected in a nuclear exchange assay at both 37 and 4 degrees C, but at neither temperature in the presence of reducing agent. Additionally, the steroid specificity of the curvilinear form was identical to the hook-shaped form. The hook-shaped form was found in both immature and ovariectomized adult rats implanted for 6 days with an E2-releasing Silastic capsule to provide pharmacological E2 levels. When uteri from implanted animals displaying the hook-shaped form were mixed in various ratios with uteri lacking type II sites, the curvilinear form was produced. An...Continue Reading

References

Mar 1, 1975·Journal of Steroid Biochemistry·E R DeSombreE V Jensen
Apr 1, 1979·Journal of Steroid Biochemistry·C R LyttleE R DeSombre
Dec 1, 1979·Endocrinology·S T Pacold, W G Blackard
Mar 15, 1978·Biochemical and Biophysical Research Communications·H ErikssonJ H Clark
Apr 1, 1969·Biology of Reproduction·J Brökelmann, D W Fawcett
Oct 12, 1973·Science·E V Jensen, E R DeSombre
Nov 1, 1981·Biology of Reproduction·W J KingE R DeSombre
Jul 1, 1981·Endocrinology·D M SheehanD R Zehr
Jan 1, 1980·Journal of Receptor Research·C S Watson, J H Clark
Dec 1, 1981·Journal of Steroid Biochemistry·W B PankoJ H Clark
Dec 1, 1981·Journal of Steroid Biochemistry·J H Clark, B M Markaverich

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Citations

Jan 1, 1994·Archives of Orthopaedic and Trauma Surgery·M IshibeK Kaneda
Nov 1, 1993·Calcified Tissue International·M L BrandiC Gennari

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