PMID: 9526507Apr 4, 1998Paper

Identification of UV/blue light-response elements in the Arabidopsis thaliana chalcone synthase promoter using a homologous protoplast transient expression system

Plant Molecular Biology
U HartmannBernd Weisshaar

Abstract

To identify DNA sequences of the Arabidopsis thaliana chalcone synthase gene (CHS) concerned with induction by UV-B and UV-A/blue light, AtCHS promoter constructions were assayed by transient expression in protoplasts prepared from two different lines of cultured A. thaliana cells. The protoplasts responded similarly to A. thaliana leaf tissue in light-dependent CHS transcript accumulation. The reporter enzyme beta-glucuronidase (GUS) was used to monitor light-responsive promoter activity. A 1972 bp promoter conferred UV-B and UV-A/blue light induction of GUS activity. Deletion to 164 bp resulted in reduced promoter strength but retention of responsiveness to UV-B and UV-A/blue light. Further deletion abolished transcriptional activity. The 164 bp promoter contains sequences closely resembling LRUPcCHS, (light-responsive unit of the Petroselinum crispum CHS promoter). This A. thaliana CHS promoter region, designated LRUAtCHS, was sufficient to confer UV-B and UV-A/blue light responsiveness to a heterologous core promoter. Mutation of sequences in LRUAtCHS corresponding to the ACGT element and the MYB recognition element of LRUPcCHS resulted in inactivation of the 164 bp and 335 bp promoter deletions. However, the mutant 668 bp ...Continue Reading

Citations

Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·E WismanB Weisshaar
Feb 13, 2008·The Plant Journal : for Cell and Molecular Biology·Judit SafranyFerenc Nagy
Apr 30, 2009·Annual Review of Plant Biology·Gareth I Jenkins
Jan 12, 2010·The Plant Journal : for Cell and Molecular Biology·María Lorena Falcone FerreyraErich Grotewold
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May 7, 2009·Nucleic Acids Research·Leonardo AlvesThomas Merkle
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Feb 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Elke Logemann, Klaus Hahlbrock
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Mar 1, 2020·Science China. Life Sciences·Feng ZhangJie Luo

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