Identification of VAPA and VAPB as Kv2 Channel-Interacting Proteins Defining Endoplasmic Reticulum-Plasma Membrane Junctions in Mammalian Brain Neurons

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Michael KirmizJames S Trimmer

Abstract

Membrane contacts between endoplasmic reticulum (ER) and plasma membrane (PM), or ER-PM junctions, are ubiquitous in eukaryotic cells and are platforms for lipid and calcium signaling and homeostasis. Recent studies have revealed proteins crucial to the formation and function of ER-PM junctions in non-neuronal cells, but little is known of the ER-PM junctions prominent in aspiny regions of mammalian brain neurons. The Kv2.1 voltage-gated potassium channel is abundantly clustered at ER-PM junctions in brain neurons and is the first PM protein that functions to organize ER-PM junctions. However, the molecular mechanism whereby Kv2.1 localizes to and remodels these junctions is unknown. We used affinity immunopurification and mass spectrometry-based proteomics on brain samples from male and female WT and Kv2.1 KO mice and identified the resident ER vesicle-associated membrane protein-associated proteins isoforms A and B (VAPA and VAPB) as prominent Kv2.1-associated proteins. Coexpression with Kv2.1 or its paralog Kv2.2 was sufficient to recruit VAPs to ER-PM junctions. Multiplex immunolabeling revealed colocalization of Kv2.1 and Kv2.2 with endogenous VAPs at ER-PM junctions in brain neurons from male and female mice in situ and i...Continue Reading

Citations

Aug 10, 2018·Molecular Biology of the Cell·Michael KirmizJames S Trimmer
Jul 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Chung-Yang YehCarlos J Camacho
May 31, 2019·The Journal of Physiology·Shannon H RomerRobert E W Fyffe
Aug 3, 2019·The Journal of General Physiology·Daisuke SatoL Fernando Santana
Sep 22, 2019·International Journal of Molecular Sciences·Cosmin I CiotuMichael J M Fischer
Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Samantha C O'DwyerL Fernando Santana
Apr 22, 2020·Cellular and Molecular Life Sciences : CMLS·Celso Almeida, Margarida D Amaral
Apr 17, 2020·Frontiers in Molecular Neuroscience·Panpan WangWeidong Le
Aug 29, 2020·International Journal of Molecular Sciences·Chung-Yang YehElias Aizenman
May 8, 2020·Scientific Reports·Tomoyuki YamanakaNobuyuki Nukina
Aug 15, 2020·Frontiers in Cell and Developmental Biology·Mohammad F ZamanChristopher T Beh
Mar 23, 2019·The Journal of Physiological Sciences : JPS·Mayra Delgado-Ramírez, Aldo A Rodríguez-Menchaca
Feb 20, 2020·Nature Reviews. Molecular Cell Biology·Marta GiacomelloLuca Scorrano
Jan 16, 2021·The Journal of Physiology·Adam S DeardorffRobert E W Fyffe
Sep 19, 2020·Current Opinion in Physiology·Tamas BallaJoshua Pemberton
Feb 24, 2020·Current Opinion in Cell Biology·Christopher J Stefan
Feb 23, 2021·Frontiers in Cell and Developmental Biology·Chenlu LiHaijia Yu
Apr 24, 2021·The Journal of Cell Biology·Andrés Guillén-SamanderPietro De Camilli
May 2, 2021·Current Opinion in Cell Biology·Rossella VendittiMaria Antonietta De Matteis
Aug 28, 2021·Cells·Nica BorgeseFrancesca Navone
Sep 29, 2021·The Journal of General Physiology·Parashar ThapaJon T Sack
Nov 18, 2021·The Journal of Cell Biology·Christopher PtakRichard W Wozniak
Jan 13, 2022·The Journal of Cell Biology·Suzan KorsMichael Schrader

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