Identification of various cell culture models for the study of Zika virus

World Journal of Virology
Kiyoshi Himmelsbach, Eberhard Hildt

Abstract

To identify cell culture models supportive for Zika virus (ZIKV) replication. Various human and non-human cell lines were infected with a defined amount of ZIKV Polynesia strain. Cells were analyzed 48 h post infection for the amount of intracellular and extracellular viral genomes and infectious viral particles by quantitative real-time PCR and virus titration assay. The extent of replication was monitored by immunofluorescence and western blot analysis by using Env and NS1 specific antibodies. Innate immunity was assayed by luciferase reporter assay and immunofluorescence analysis. All investigated cell lines except CHO cells supported infection, replication and release of ZIKV. While in infected A549 and Vero cells a pronounced cytopathic effect was observed COS7, 293T and Huh7.5 cells were most resistant. Although the analyzed cell lines released comparable amounts of viral genomes to the supernatant significant differences were found for the number of infectious viral particles. The neuronal cell lines N29.1 and SH-SY5Y released 100 times less infectious viral particles than Vero-, A549- or 293T-cells. However there is no strict correlation between the amount of produced viral particles and the induction of an interferon r...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Jul 1, 1987·Journal of Clinical Microbiology·L A White
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·J G Olson Triwibowo
Nov 20, 2002·Journal of Virology·Keril J BlightCharles M Rice
Jun 12, 2009·The New England Journal of Medicine·Mark R DuffyEdward B Hayes
Oct 1, 2009·Emerging Infectious Diseases·Edward B Hayes
Oct 31, 2012·European Journal of Cell Biology·Kiyoshi Himmelsbach, Eberhard Hildt
Mar 15, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·E OehlerF Ghawche
Jun 19, 2015·Journal of Virology·Rodolphe HamelDorothée Missé
Jan 14, 2016·The New England Journal of Medicine·Anthony S Fauci, David M Morens
Feb 11, 2016·The New England Journal of Medicine·Jernej MlakarTatjana Avšič Županc
Feb 22, 2016·The Lancet Infectious Diseases·Guilherme CalvetAna M B de Filippis
Mar 2, 2016·Journal of Infection in Developing Countries·Jorg HeukelbachLuciano Pamplona de Góes Cavalcanti
Mar 31, 2016·The New England Journal of Medicine·Rita W DriggersOlli Vapalahti
Apr 14, 2016·Cell Host & Microbe·Helen M LazearMichael S Diamond
May 7, 2016·PLoS Neglected Tropical Diseases·Stuart D DowallRoger Hewson
Aug 20, 2016·PLoS Neglected Tropical Diseases·Kelli L BarrMaureen T Long
Oct 21, 2016·Scientific Reports·Hicham El CostaNabila Jabrane-Ferrat
Nov 1, 2016·EMBO Reports·Anil KumarTom C Hobman
Dec 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Hanna RetallackJoseph L DeRisi
Mar 2, 2017·Cell Reports·Mirko CorteseRalf Bartenschlager
Apr 27, 2017·Proceedings of the National Academy of Sciences of the United States of America·Qian ZhangAlessandro Vespignani
Jun 26, 2017·Lancet·David BaudDidier Musso

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Citations

Mar 28, 2018·Viruses·Fabian ElgnerEberhard Hildt
May 17, 2019·Reviews in Medical Virology·Sangya Shaily, Archana Upadhya
Jul 25, 2019·Frontiers in Microbiology·Svetlana F KhaiboullinaSubhash C Verma
Apr 12, 2019·Frontiers in Cellular Neuroscience·Christian AlfanoLaurent Nguyen
Mar 8, 2019·PLoS Neglected Tropical Diseases·Kenneth H Dinnon IiiRalph S Baric
Jul 6, 2019·Virus Evolution·Cory D DuPaiClaus O Wilke
Sep 10, 2021·Journal of Medical Microbiology·Fernando Ruiz-JiménezJonathan K Ball

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Datasets Mentioned

BETA
AB16951

Methods Mentioned

BETA
PCR
electrophoresis
X-ray
transfection
protein assay

Software Mentioned

Image J
ZEN
Prism GraphPad

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