Identification of vitreous proteins in retinopathy of prematurity

Biochemical and Biophysical Research Communications
Koji SugiokaYoshikazu Shimomura

Abstract

Retinopathy of prematurity (ROP) is a disorder of blood vessels in the retina developed in premature infants and the leading cause of the blindness in children. Proteomic analysis was performed to identify vitreous proteins specific to patients with ROP. Vitreous humor samples were obtained from three patients with ROP and two patients with congenital cataract, the latter included as a control group. The vitreous samples were separated by 2D-PAGE and the proteins running as definitive spots were identified by MALDI-TOF MS spectrometry. We identified 13 and 6 proteins in the vitreous from ROP and cataract patients, respectively. Albumin, transferrin, pigment epithelium-derived factor (PEDF) and transthyretin were found in both patient groups. In the samples from ROP patients, PEDF and transthyretin levels were lower than in those from cataract patients, and retinol binding protein 3 and prostaglandin D synthase were not detected. Of the 13 proteins, 9 proteins including α-2-macroglobulin, ceruloplasmin, α-fetoprotein, vitamin D-binding protein, α-1-antitrypsin, α-1-β-glycoprotein, hemopexin, apolipoprotein A-1 and A-lV were found in vitreous samples of only the ROP patients. PEDF has anti-angiogenic and neurotrophic functions. W...Continue Reading

Citations

Nov 7, 2018·Investigative Ophthalmology & Visual Science·Magdalena ZasadaPrzemko Kwinta
Aug 10, 2019·Plant Signaling & Behavior·Ke Zhou
Jul 1, 2020·Frontiers in Pharmacology·Maria VähätupaHannele Uusitalo-Järvinen
Oct 3, 2018·Translational Vision Science & Technology·Gabriel VelezVinit B Mahajan
Jun 4, 2019·Analytical and Bioanalytical Chemistry·Fátima M SantosLuís A Passarinha
Jan 23, 2021·Journal of Clinical Medicine·Mitsuru ArimaKoh-Hei Sonoda
Mar 2, 2021·Investigative Ophthalmology & Visual Science·Anand VinekarSwaminathan Sethu
Mar 15, 2020·Forensic Science International : Synergy·Nicola PigaianiFranco Tagliaro
Sep 15, 2019·Experimental Eye Research·Nader SheibaniJack Henkin
Jun 3, 2021·Journal of Clinical Medicine·Sarah R WeberJeffrey M Sundstrom

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