Identification, purification and characterization of furfural transforming enzymes from Clostridium beijerinckii NCIMB 8052

Anaerobe
Yan ZhangThaddeus Chukwuemeka Ezeji

Abstract

Generation of microbial inhibitory compounds such as furfural and 5-hydroxymethylfurfural (HMF) is a formidable roadblock to fermentation of lignocellulose-derived sugars to butanol. Bioabatement offers a cost effective strategy to circumvent this challenge. Although Clostridium beijerinckii NCIMB 8052 can transform 2-3 g/L of furfural and HMF to their less toxic alcohols, higher concentrations present in biomass hydrolysates are intractable to microbial transformation. To delineate the mechanism by which C. beijerinckii detoxifies furfural and HMF, an aldo/keto reductase (AKR) and a short-chain dehydrogenase/reductase (SDR) found to be over-expressed in furfural-challenged cultures of C. beijerinckii were cloned and over-expressed in Escherichia coli Rosetta-gami™ B(DE3)pLysS, and purified by histidine tag-assisted immobilized metal affinity chromatography. Protein gel analysis showed that the molecular weights of purified AKR and SDR are close to the predicted values of 37 kDa and 27 kDa, respectively. While AKR has apparent Km and Vmax values of 32.4 mM and 254.2 mM s(-1) respectively, using furfural as substrate, SDR showed lower Km (26.4 mM) and Vmax (22.6 mM s(-1)) values on the same substrate. However, AKR showed 7.1-fol...Continue Reading

References

Mar 6, 1998·Trends in Biotechnology·G Hannig, S C Makrides
Jan 28, 1999·Biotechnology and Bioengineering·E PalmqvistB Hahn-Hägerdal
Jul 4, 2003·Applied and Environmental Microbiology·Ilona Sárvári HorváthGunnar Lidén
Jul 13, 2004·Trends in Biotechnology·Claes GustafssonJeremy Minshull
Oct 17, 2009·Biotechnology for Biofuels·Tirzah Y MillsRyan T Gill
Feb 13, 2010·Biotechnology for Biofuels·Sandra A AllenSteven W Gorsich
Jul 2, 2011·Biotechnology Letters·Manoj Agrawal, Rachel Ruizhen Chen

❮ Previous
Next ❯

Citations

Nov 26, 2015·Applied Microbiology and Biotechnology·Victor UjorThaddeus Chukwuemeka Ezeji
May 19, 2019·Applied and Environmental Microbiology·Alan F ScottRudolf K Allemann
Sep 16, 2017·Bioresources and Bioprocessing·Ping WeiWen-Yong Lou
Feb 17, 2021·Biotechnology Letters·José Eduardo ArteagaJuan-Carlos Sigala

❮ Previous
Next ❯

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.