Identification, transcription and sequence analysis of the Spodoptera littoralis nucleopolyhedrovirus (SpliNPV) DNA polymerase gene

Archives of Virology
J Huang, D B Levin

Abstract

Sequence analysis of a 6.4 kb DNA region from the Spodoptera littoralis multinucleocapsid nucleopolyhedrovirus (SpliNPV) revealed a large open reading frame (ORF) encoding a predicted polypeptide of 998 amino acid (aa) residues with a molecular mass of 114.93 kDa, located between 47.2-52.3 m.u. on the SpliNPV genome. Comparative sequence analyses demonstrated that the ORF encodes a DNA polymerase gene (dnapol) that contains conserved exonuclease domains and DNA polymerase motifs found in many prokaryotic, eukaryotic, and viral replicative DNA polymerases. A second ORF, ORF138, located between the lef-3 and dnapol, encodes a 138 aa polypetide that is homologous to ORF66 of the Autographa californica MNPV (AcMNPV). SpliNPV DNA polymerase shares an overall aa sequence identity of 39% with that of AcMNPV. A 3.0 kb SpliNPV dnapol-specific transcript was detected initially at 2 hpi and became abundant 48 hpi by Northern blot analysis. The transcription initiation site was mapped to an NPV early promoter element, ACGT. 3' RACE demonstrated that the SpliNPV dnapol transcript terminated at the polyadenylation signal AATAAA. Sequence analysis suggested that the SpliNPV dnapol and the dnapol of the NPV of S. litura (SpltNPV) are closely r...Continue Reading

Citations

Jul 10, 2008·Archives of Virology·Sally Hilton, Doreen Winstanley
Jun 1, 2005·Molecular and Biochemical Parasitology·Fiona SeowMichael P Barrett
Jul 1, 2006·Journal of Virology·Simon P DuffyDavid B Levin
Jul 24, 2008·Virus Genes·Juliana Velasco de Castro OliveiraPaolo Marinho de Andrade Zanotto
Aug 2, 2005·Journal of Biochemistry and Molecular Biology·Paresa N GiannopoulosKianoush Khajeh Rashidan

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