Identifying a Major QTL Associated with Salinity Tolerance in Nile Tilapia Using QTL-Seq

Marine Biotechnology
Xiao Hui GuJun Hong Xia

Abstract

Selection of new lines with high salinity tolerance allows for economically feasible production of tilapias in brackish water areas. Mapping QTLs and identifying the markers linked to salinity-tolerant traits are the first steps in the improvement of the tolerance in tilapia through marker-assisted selection techniques. By using QTL-seq strategy and linkage-based analysis, two significant QTL intervals (chrLG4 and chrLG18) on salinity-tolerant traits were firstly identified in the Nile tilapia. Fine mapping with microsatellite and SNP markers suggested a major QTL region that located at 23.0 Mb of chrLG18 and explained 79% of phenotypic variation with a LOD value of 95. Expression analysis indicated that at least 10 genes (e.g., LACTB2, KINH, NCOA2, DIP2C, LARP4B, PEX5R, and KCNJ9) near or within the QTL interval were significantly differentially expressed in intestines, brains, or gills under 10, 15, or 20 ppt challenges. Our findings suggest that QTL-seq can be effectively utilized in QTL mapping of salinity-tolerant traits in fish. The identified major QTL is a promising locus to improve our knowledge on the genetic mechanism of salinity tolerance in tilapia.

References

Nov 12, 2003·The Journal of Experimental Biology·Jeff G RichardsPatricia M Schulte
Sep 28, 2004·Molecular Genetics and Genomics : MGG·A CnaaniM Ron
Jun 11, 2005·BMC Genomics·Takayuki KatagiriThomas D Kocher
Jan 13, 2006·General and Comparative Endocrinology·Tatsuya Sakamoto, Stephen D McCormick
Aug 22, 2006·Nature Reviews. Molecular Cell Biology·Jesús Gil, Gordon Peters
Feb 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Emilie FarcyJean-Marc Lebel
Mar 7, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C K TipsmarkS S Madsen
Aug 12, 2008·The Journal of Experimental Biology·Junya HiroiToyoji Kaneko
Dec 1, 2009·The Journal of Experimental Biology·S D McCormickA K Christensen
Dec 4, 2010·Biochimica Et Biophysica Acta·Diego F FiolDietmar Kültz
Sep 23, 2011·BMC Genetics·Joseph D NormanMoira M Ferguson
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Mar 17, 2012·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Catherine Lorin-NebelJean-Dominique Durand
Apr 25, 2012·Molecular Ecology Resources·N E M Van BersJ Komen
Jul 31, 2012·Biochemical and Biophysical Research Communications·Biao YanJin-Liang Zhao
Aug 10, 2012·Physiology·Dietmar Kültz
Jan 8, 2013·The Plant Journal : for Cell and Molecular Biology·Hiroki TakagiRyohei Terauchi
Sep 26, 2013·Molecular & Cellular Proteomics : MCP·Dietmar KültzRomina Sacchi
Sep 1, 1992·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·J W van Ooijen
Sep 5, 2014·Nature·David BrawandFederica Di Palma
Apr 30, 2015·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Shouvik DasSwarup K Parida
Sep 17, 2015·Scientific Reports·Jun Hong XiaGen Hua Yue
Feb 9, 2017·Marine Biotechnology·Bi Jun LiJun Hong Xia
Mar 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Xiaodan Wang, Dietmar Kültz
Apr 15, 2017·Zoological Research·Yan-Bo Sun

❮ Previous
Next ❯

Citations

Apr 18, 2020·Nature Reviews. Genetics·Ross D HoustonDiego Robledo
Mar 14, 2019·Marine Biotechnology·Bi Jun LiJun Hong Xia

❮ Previous
Next ❯

Datasets Mentioned

BETA
SSR448

Methods Mentioned

BETA
RNA-seq
Electrophoresis
PCR

Software Mentioned

FasParser
Excel
JoinMap
BLAST
bowtie2
MapQTL
Primer
CodonCode Aligner

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.