Identifying a Minor Histocompatibility Antigen in Mauritian Cynomolgus Macaques Encoded by APOBEC3C.

Frontiers in Immunology
J. T. WeinfurterMatthew R Reynolds

Abstract

Allogeneic hematopoietic stem cell transplants can lead to dramatic reductions in human immunodeficiency virus (HIV) reservoirs. This effect is partially mediated by donor T cells recognizing lymphocyte-expressed minor histocompatibility antigens (mHAgs). The potential to mark malignant and latently infected cells for destruction makes mHAgs attractive targets for cellular immunotherapies. However, testing such HIV reservoir reduction strategies will likely require preclinical studies in non-human primates (NHPs). In this study, we used a combination of alloimmunization, whole exome sequencing, and bioinformatics to identify an mHAg in Mauritian cynomolgus macaques (MCMs). We mapped the minimal optimal epitope to a 10-mer peptide (SW10) in apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3C (APOBEC3C) and determined the major histocompatibility complex class I restriction element as Mafa-A1∗063, which is expressed in almost 90% of MCMs. APOBEC3C SW10-specific CD8+ T cells recognized immortalized B cells but not fibroblasts from an mHAg-positive MCM. These results provide a framework for identifying mHAgs in a non-transplant setting and suggest that APOBEC3C SW10 could be used as a model antigen to test mHAg-targe...Continue Reading

References

Feb 1, 1995·American Journal of Physical Anthropology·S H LawlerL L Taylor
Nov 1, 1996·European Journal of Immunology·J M den HaanE Goulmy
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·T W ChunA S Fauci
Nov 30, 1999·Nature·T W ChunA S Fauci
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·R T DaveyH C Lane
Dec 18, 2003·AIDS Research and Human Retroviruses·Jonathan G LeithKelly S MacDonald
Mar 27, 2007·Immunogenetics·Shelby L O'ConnorDavid H O'Connor
Feb 14, 2009·The New England Journal of Medicine·Gero HütterEckhard Thiel
Jul 10, 2009·Journal of Virology·Fransje A KoningMichael H Malim
Jan 14, 2011·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·J H Frederik Falkenburg, Edus H Warren
Feb 9, 2011·Immunology and Cell Biology·Marie Bleakley, Stanley R Riddell
Jul 8, 2011·Blood·Krystel VincentClaude Perreault
Jul 21, 2012·Nature Reviews. Immunology·Steven G DeeksFrançoise Barré-Sinoussi
Mar 5, 2013·Current Opinion in HIV and AIDS·Ronald Mitsuyasu
Jun 13, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Eric SpieringsEls Goulmy
Oct 29, 2013·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Krystel VincentClaude Perreault
Nov 1, 2013·ILAR Journal·Roger W WisemanDavid H O'Connor
Aug 26, 2014·Haematologica·Rimke OostvogelsRobbert M Spaapen
Apr 17, 2015·The Journal of Infectious Diseases·Amanda M CrooksNancie M Archin
Mar 26, 2016·Frontiers in Immunology·Marieke GriffioenJ H Frederik Falkenburg
Sep 15, 2016·AIDS Research and Therapy·Gero Hütter
Dec 23, 2016·Archives of Gynecology and Obstetrics·Marcelo Borges CavalcanteRicardo Barini
Mar 30, 2017·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Kersten K KoelschDavid A Cooper
Jun 24, 2017·Frontiers in Immunology·Anne M DickinsonHans Jochem-Kolb
Nov 21, 2017·Cell Chemical Biology·Margaret E OlsonDaniel A Harki

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Methods Mentioned

BETA
cDNA
density gradient centrifugation
FCS
FACS
exome sequencing

Software Mentioned

Variant Segregator
Prism
OSX
GraphPad
FlowJo

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