Identifying a Molecular Mechanism That Imparts Species-Specific Toxicity to YoeB Toxins

Frontiers in Microbiology
Jessica R AmesChristina R Bourne

Abstract

The ribosome-dependent E. coli (Ec) mRNase toxin YoeB has been demonstrated to protect cells during thermal stress. Agrobacterium tumefaciens (At), a plant pathogen, also encodes a YoeB toxin. Initial studies indicated that AtYoeB does not impact the growth of Ec, but its expression is toxic to the native host At. The current work examines this species-specific effect. We establish the highly similar structure and function of Ec and AtYoeB toxins, including the ability of the AtYoeB toxin to inhibit Ec ribosomes in vitro. Comparison of YoeB sequences and structures highlights a four-residue helix between β-strands 2 and 3 that interacts with mRNA bases within the ribosome. This helix sequence is varied among YoeB toxins, and this variation correlates with bacterial classes of proteobacteria. When the four amino acid sequence of this helix is transplanted from EcYoeB onto AtYoeB, the resulting chimera gains toxicity to Ec cells and lessens toxicity to At cells. The reverse is also true, such that EcYoeB with the AtYoeB helix sequence is less toxic to Ec and gains toxicity to At cultures. We suggest this helix sequence directs mRNA sequence-specific degradation, which varies among proteobacterial classes, and thus controls growth...Continue Reading

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K GerdesS Molin
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T Ogura, S Hiraga
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·H Lehnherr, M B Yarmolinsky
Feb 1, 1993·Molecular & General Genetics : MGG·S Tsuchimoto, E Ohtsubo
Jun 18, 2002·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·Ashkan GolshaniIvan G Ivanov
Jun 6, 2003·Molecular Microbiology·Susanne K Christensen, Kenn Gerdes
Jun 3, 2004·Genome Research·Gavin E CrooksSteven E Brenner
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Feb 19, 2005·Nucleic Acids Research·Deo Prakash Pandey, Kenn Gerdes
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Jul 10, 2007·Journal of Bacteriology·Roy David Magnuson
Aug 7, 2007·Genome Biology·Emeric W Sevin, Frédérique Barloy-Hubler
Jun 6, 2008·Molecular Microbiology·Martin OvergaardKenn Gerdes
Jan 7, 2009·The Journal of Biological Chemistry·Yonglong Zhang, Masayori Inouye
Mar 28, 2009·PLoS Genetics·Laurence Van Melderen, Manuel Saavedra De Bast
Mar 28, 2009·PLoS Genetics·Rachel A F Wozniak, Matthew K Waldor
May 9, 2009·The Journal of Biological Chemistry·Jennifer M Hurley, Nancy A Woychik
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jul 8, 2009·Journal of Bacteriology·Satoshi YoshizumiMasayori Inouye
Dec 30, 2009·Journal of Bacteriology·Niles P DoneganAmbrose L Cheung
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Nov 3, 2010·Current Opinion in Microbiology·Laurence Van Melderen
Jul 19, 2011·Journal of Bacteriology·Wai Ting ChanChew Chieng Yeo
Apr 17, 2012·Acta Crystallographica. Section D, Biological Crystallography·Pavel V AfoninePaul D Adams
Feb 12, 2013·Plasmid·Iwona Brzozowska, Urszula Zielenkiewicz
Nov 20, 2013·Mobile Genetic Elements·Simon J UnterholznerWilfried Rozhon
Apr 23, 2014·Nucleic Acids Research·Xavier Robert, Patrice Gouet
Aug 15, 2014·Biochemical and Biophysical Research Communications·Tauqeer AhmadMounir G AbouHaidar
Jul 7, 2015·MicrobiologyOpen·Brian D JanssenChristopher S Hayes
Aug 12, 2015·Nucleic Acids Research·Tatsuya MaehigashiChristine M Dunham

❮ Previous
Next ❯

Citations

Feb 13, 2021·Scientific Reports·Julia McGillickChristina R Bourne

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
Assay
PCR
Light
size exclusion chromatography
light scattering
Differential Scanning Fluorimetry

Key Resources (RRID) Mentioned

Addgene_60733

Software Mentioned

CCP4
WebLogo
PHASER
ASTRA
Phenix
AIMLESS
Rapid Automated Scan for Toxins and Antitoxins in Bacteria ( R...
COOT
ESpript
UCSF Chimera

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.