Identifying cancer origin using circulating tumor cells

Cancer Biology & Therapy
Si-Hong LuYing-Chih Chang

Abstract

Circulating tumor cells (CTCs) have become an established clinical evaluation biomarker. CTC count provides a good correlation with the prognosis of cancer patients, but has only been used with known cancer patients, and has been unable to predict the origin of the CTCs. This study demonstrates the analysis of CTCs for the identification of their primary cancer source. Twelve mL blood samples were equally dispensed on 6 CMx chips, microfluidic chips coated with an anti-EpCAM-conjugated supported lipid bilayer, for CTC capture and isolation. Captured CTCs were eluted to an immunofluorescence (IF) staining panel consisting of 6 groups of antibodies: anti-panCK, anti-CK18, anti-CK7, anti-TTF-1, anti-CK20/anti-CDX2, and anti-PSA/anti-PSMA. Cancer cell lines of lung (H1975), colorectal (DLD-1, HCT-116), and prostate (PC3, DU145, LNCaP) were selected to establish the sensitivity and specificity for distinguishing CTCs from lung, colorectal, and prostate cancer. Spiking experiments performed in 2mL of culture medium or whole blood proved the CMx platform can enumerate cancer cells of lung, colorectal, and prostate. The IF panel was tested on blood samples from lung cancer patients (n = 3), colorectal cancer patients (n = 5), prostate ...Continue Reading

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Citations

Jul 4, 2016·Bulletin du cancer·Marc-Antoine BenderraChristophe Massard
Feb 28, 2018·Journal of Experimental & Clinical Cancer Research : CR·Y T LuoQ Huang
Aug 7, 2019·Future Oncology·Elie RassyNicholas Pavlidis
Jun 20, 2020·Proteomics. Clinical Applications·Chen ChenHans Neubauer
Sep 6, 2018·Therapeutic Advances in Medical Oncology·Raffaele PalmirottaFranco Silvestris
Jan 29, 2021·Cancers·Noemi LaproviteraManuela Ferracin
May 1, 2021·Cancers·Kalyani B KarunakaranMadhavi K Ganapathiraju
Jun 10, 2019·ACS Biomaterials Science & Engineering·Yu-Lin TsaiYing-Chih Chang

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Methods Mentioned

BETA
biopsy
chip
chips

Software Mentioned

Elements AR Analysis
SPSS
CMx
CellSearch→
NIS

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