Identifying FGA peptides as nasopharyngeal carcinoma-associated biomarkers by magnetic beads

Journal of Cellular Biochemistry
Ya-Lan TaoYun-Fei Xia

Abstract

Early diagnosis and treatment is known to improve prognosis for nasopharyngeal carcinoma (NPC). The study determined the specific peptide profiles by comparing the serum differences between NPC patients and healthy controls, and provided the basis for the diagnostic model and identification of specific biomarkers of NPC. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) can be used to detect the molecular mass of peptides. Mass spectra of peptides were generated after extracting and purification of 40 NPC samples in the training set, 21 in the single center validation set and 99 in the multicenter validation set using weak cationic-exchanger magnetic beads. The spectra were analyzed statistically using FlexAnalysis™ and ClinProt™ bioinformatics software. The four most significant peaks were selected out to train a genetic algorithm model to diagnose NPC. The diagnostic sensitivity and specificity were 100% and 100% in the training set, 90.5% and 88.9% in the single center validation set, 91.9% and 83.3% in the multicenter validation set, and the false positive rate (FPR) and false negative rate (FNR) were obviously lower in the NPC group (FPR, 16.7%; FNR, 8.1%) than in the other cancer ...Continue Reading

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Citations

Jul 18, 2015·International Journal of Molecular Sciences·Ze-Tan ChenXiao-Dong Zhu
Jun 20, 2015·BioMed Research International·Tingting AiJiuxiang Lin
May 23, 2015·Disease Markers·Tavan Janvilisri
Mar 13, 2014·Expert Review of Proteomics·Liang XiaoZhi-Qiang Xiao
May 7, 2019·Expert Review of Proteomics·Zhefeng Xiao, Zhuchu Chen
Jul 6, 2021·Frontiers in Oncology·Julia EberleLisa Wiesmüller

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