Identifying HIV-1 host cell factors by genome-scale RNAi screening

Methods : a Companion to Methods in Enzymology
Lars PacheSumit K Chanda

Abstract

Advances in the application of RNA interference (RNAi) have facilitated the establishment of systematic cell-based loss-of-function screening platforms. Widespread implementation of this technology has enabled genome-wide genetic analysis of a diverse array of cellular phenotypes, including the identification of host cell factors involved in viral replication. Four recent studies employed whole-genome RNAi technologies to elucidate cellular genes important for the replication of HIV-1. While these four genome-scale screens shared a common objective, they differ in their scope and experimental design. In this review we explore alternative strategies for developing RNAi screens, and discuss potential pitfalls of the technology. Important technical considerations include the choice of silencing reagents, experimental systems, assay readout and analysis methods. We focus on experimental and computational parameters that can impact the outcome of high-throughput genetic screens, and provide guidelines for the development of reliable cell-based RNAi screens.

References

Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
May 29, 2003·Nucleic Acids Research·Frank CzaudernaJörg Kaufmann
Feb 7, 2004·Nature·Tao LiDarrel W Stafford
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Sep 9, 2006·Science·Manuel LlanoEric M Poeschla
Oct 13, 2007·Nature Reviews. Drug Discovery·Charles Flexner
Nov 4, 2008·Cell Host & Microbe·Honglin ZhouAmy S Espeseth
Nov 6, 2008·Cell·Stephen P Goff
Aug 29, 2009·Reviews in Medical Virology·Erik De Clercq

❮ Previous
Next ❯

Citations

May 13, 2014·PloS One·Dorothea Emig-AgiusTrey Ideker
Jul 13, 2011·International Journal of Molecular Sciences·Andrew PrussiaRichard K Plemper
Dec 14, 2011·Current Opinion in HIV and AIDS·Helder I Nakaya, Bali Pulendran
Aug 7, 2012·Expert Opinion on Drug Discovery·Susanne Heynen-GenelJonathan Rosen
Nov 6, 2012·Current Opinion in Virology·Debasis Panda, Sara Cherry
Jan 22, 2011·Methods : a Companion to Methods in Enzymology·Melanie Ott
Jun 27, 2015·Current Opinion in Microbiology·Renate König, Silke Stertz
Feb 12, 2013·New Biotechnology·Simone C Eicher, Christoph Dehio
Mar 15, 2015·AIDS Research and Human Retroviruses·Corine St GelaisLi Wu
Dec 24, 2014·BMC Genomics·Pauli RämöChristoph Dehio
May 13, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Helder I Nakaya, Bali Pulendran
Jan 17, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Victoria A MeliopoulosRalph A Tripp
May 15, 2020·Clinical Microbiology Reviews·Naveen KumarBarry T Rouse

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell-Type-Specific Viral Vectors (ASM)

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Cell-Type Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.

Cell-Type-Specific Viral Vectors

Viral vectors are used in biological research and therapy to deliver genetic material into cells. However, the efficiency of viral vectors varies depending on the cell type. Here is the latest research on cell-type-specific viral vectors.