Identifying Isl1 Genetic Lineage in the Developing Olfactory System and in GnRH-1 Neurons

Frontiers in Physiology
Ed Zandro M TarocPaolo E Forni

Abstract

During embryonic development, symmetric ectodermal thickenings [olfactory placodes (OP)] give rise to several cell types that comprise the olfactory system, such as those that form the terminal nerve ganglion (TN), gonadotropin releasing hormone-1 neurons (GnRH-1ns), and other migratory neurons in rodents. Even though the genetic heterogeneity among these cell types is documented, unidentified cell populations arising from the OP remain. One candidate to identify placodal derived neurons in the developing nasal area is the transcription factor Isl1, which was recently identified in GnRH-3 neurons of the terminal nerve in fish, as well as expression in neurons of the nasal migratory mass (MM). Here, we analyzed the Isl1 genetic lineage in chemosensory neuronal populations in the nasal area and migratory GnRH-1ns in mice using in situ hybridization, immunolabeling a Tamoxifen inducible Isl1CreERT and a constitutive Isl1Cre knock-in mouse lines. In addition, we also performed conditional Isl1 ablation in developing GnRH neurons. We found Isl1 lineage across non-sensory cells of the respiratory epithelium and sustentacular cells of OE and VNO. We identified a population of transient embryonic Isl1 + neurons in the olfactory epithel...Continue Reading

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Citations

Apr 20, 2021·Cellular and Molecular Life Sciences : CMLS·Raghu Ram Katreddi, Paolo E Forni
Sep 24, 2021·Journal of Neuroendocrinology·Yufei Shan, Susan Wray

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Methods Mentioned

BETA
transgenic
in vitro transcription

Software Mentioned

FIJ
ImageJ
GraphPad Prism7

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