Identifying miltefosine-resistant key genes in protein-protein interactions network and experimental verification in Iranian Leishmania major

Molecular Biology Reports
Niloofar LariMajid Rajabian Noghondar

Abstract

Drug resistance is a complex phenomenon during leishmaniasis chemotherapy. In this study, the genes and pathways involved in miltefosine (MIL)-resistant Leishmania were identified using microarray data and in silico approaches. GSE30685 and GSE45496 were obtained from GEO database and analyzed with GEO2R tool to identify genes involved in MIL-resistant Leishmania. 177 differentially expressed genes (DEGs) were selected from these GSEs, which about half of them were uncharacterized/hypothetical proteins. The interactions between DEGs were investigated using STRING database and protein-protein interaction (PPI) networks. Five hub nodes were found in the PPI network. The gene ontology (GO) analysis of the resulting network revealed that DNA replication (GO:0006260) and ATP hydrolysis coupled proton transport (GO:0015991) were the most enriched GO term. Iranian MIL-resistant Leishmania major (L. major) parasites were generated by exposure of wild-type isolates to the increasing concentrations of MIL over a period of 5 months. Proof of mRNA expression levels of the obtained hub genes was assessed in Iranian wild-type and acquired resistant L. major parasites by real-time PCR. A significant higher expression level of LDBPK_150170 (en...Continue Reading

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Citations

Jul 27, 2021·Parasitology Research·Jenny Nancy Gómez-SandovalM Magdalena Aguirre-García

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Datasets Mentioned

BETA
GPL13919
GSE30685
GSE45496

Methods Mentioned

BETA
PCR
chips
Feature extraction

Software Mentioned

GENESPRING GX
Blast p
STRING
DAVID
Blast
BLASTx
Cytoscape
PSORTb
Primer
ClusterOne

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