Identifying numerically abundant culturable bacteria from complex communities: an example from a lignin enrichment culture.

Applied and Environmental Microbiology
J M GonzálezM A Moran

Abstract

Culturable bacteria that were numerically important members of a marine enrichment community were identified and characterized phylogenetically. Selective and nonselective isolation methods were used to obtain 133 culturable bacterial isolates from model marine communities enriched with the high-molecular-weight (lignin-rich) fraction of pulp mill effluent. The culture collection was screened against community DNA from the lignin enrichments by whole-genome hybridization methods, and three marine bacterial isolates were identified as being numerically important in the communities. One isolate was in the alpha-subclass of Proteobacteria, and the other two were in the gamma-subclass of Proteobacteria. Isolate-specific 16S rRNA oligonucleotide probes designed to precisely quantify the isolates in the lignin enrichment communities indicated contributions ranging from 2 to 32% of enrichment DNA, values nearly identical to those originally obtained by the simpler whole-genome hybridization method. Two 16S rRNA sequences closely related to that of one of the isolates, although not identical, were amplified via PCR from the seawater sample originally used to inoculate the enrichment medium. Partial sequences of 14 other isolates reveal...Continue Reading

References

May 1, 1977·Applied and Environmental Microbiology·J E HobbieS Jasper
Mar 12, 1992·Nature·J A FuhrmanA A Davis
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E F DeLong
Apr 1, 1991·Applied and Environmental Microbiology·Y L Tsai, B H Olson
Jun 1, 1991·Applied and Environmental Microbiology·T B Britschgi, S J Giovannoni
Jul 1, 1991·Journal of Bacteriology·T M SchmidtN R Pace
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 3, 1990·Nature·S J GiovannoniK G Field
Jun 1, 1985·Applied and Environmental Microbiology·D A StahlN R Pace
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Mar 1, 1985·Journal of Clinical Microbiology·P A GrimontP Tchen
Oct 1, 1993·Applied and Environmental Microbiology·E B Braun-HowlandS A Nierzwicki-Bauer
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·S M BarnsN R Pace
Sep 1, 1994·Nucleic Acids Research·B L MaidakC R Woese
May 1, 1993·Applied and Environmental Microbiology·J A FuhrmanA A Davis
Oct 20, 1994·Nature·E F DeLongR V Jovine
Jun 1, 1988·Applied and Environmental Microbiology·J A FuhrmanA M Chan
Mar 1, 1993·Applied and Environmental Microbiology·D K ButtonB R Robertson
Sep 1, 1994·Microbial Ecology·J A FuhrmanR M Wilcox

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Citations

Jul 6, 2000·Applied and Environmental Microbiology·H EilersR Amann
Oct 31, 2000·Applied and Environmental Microbiology·H EilersR Amann
Feb 24, 2001·Environmental Microbiology·B D LanoilS J Giovannoni
Oct 31, 2002·Applied and Environmental Microbiology·Lasse Riemann, Farooq Azam
Oct 6, 2005·Applied and Environmental Microbiology·Alison BuchanMary Ann Moran
Dec 3, 2011·FEMS Microbiology Ecology·Dagmar RockerMeinhard Simon
Oct 26, 2014·Applied Microbiology and Biotechnology·Jiang-Hao TianPascal Peu
Jan 5, 2002·Applied and Environmental Microbiology·Veljo KisandJohan Wikner
Apr 20, 2001·FEMS Microbiology Ecology·H U. UphoffI Wagner-Döbler
Oct 6, 2009·Applied and Environmental Microbiology·Alison BuchanMarcelino T Suzuki
Jun 5, 2015·Frontiers in Microbiology·Grace PoldKristen M DeAngelis
May 1, 2016·Applied and Environmental Microbiology·Sonia GiubergiaLone Gram
Aug 21, 2019·Nature Microbiology·Beth T WilliamsJonathan D Todd
Sep 7, 2000·Microbiology and Molecular Biology Reviews : MMBR·A T BullM Goodfellow
Oct 27, 2001·Applied and Environmental Microbiology·A E AllenS Zani
Nov 28, 2001·Applied and Environmental Microbiology·A BuchanM A Moran
Apr 7, 2005·Applied and Environmental Microbiology·Cecilia Alonso, Jakob Pernthaler
Dec 8, 2005·FEMS Microbiology Ecology·Heike StevensThorsten Brinkhoff
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Ilse VandecandelaerePeter Vandamme
Apr 10, 2019·Applied Microbiology and Biotechnology·Daniel P BrinkMarie F Gorwa-Grauslund
Mar 10, 2004·Applied and Environmental Microbiology·Alison BuchanMary Ann Moran
Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·José M GonzálezMary Ann Moran
Feb 19, 2011·Annual Review of Marine Science·Elizabeth B Kujawinski
Sep 23, 2018·Applied and Environmental Microbiology·Ashley M FrankAlison Buchan

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