Identifying Protein Conformational Dynamics Using Spin-label ESR

Chemistry, an Asian Journal
Yei-Chen LaiYun-Wei Chiang

Abstract

Spin-label electron spin resonance (ESR) has emerged as a powerful tool to characterize protein dynamics. One recent advance is the development of ESR for resolving dynamical components that occur or coexist during a biological process. It has been applied to study the complex structural and dynamical aspects of membranes and proteins, such as conformational changes in protein during translocation from cytosol to membrane, conformational exchange between equilibria in response to protein-protein and protein-ligand interactions in either soluble or membrane environments, protein oligomerization, and temperature- or hydration-dependent protein dynamics. As these topics are challenging but urgent for understanding the function of a protein on the molecular level, the newly developed ESR methods to capture individual dynamical components, even in low-populated states, have become a great complement to other existing biophysical tools.

References

Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C AltenbachW L Hubbell
Apr 12, 2002·Journal of Magnetic Resonance·Gunnar JeschkeAdelheid Godt
Jan 15, 2005·Journal of Magnetic Resonance·Yun-Wei ChiangJack H Freed
Jul 5, 2005·Biophysical Journal·Christian AltenbachWayne L Hubbell
May 11, 2006·Chemical Reviews·Kazuyuki Akasaka
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·S-H ChenE Mamontov
Sep 5, 2006·Current Opinion in Structural Biology·Gail E Fanucci, David S Cafiso
Sep 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Weihong QiuAhmed H Zewail
Jul 5, 2007·Methods in Enzymology·Peter P Borbat, Jack H Freed
Jun 25, 2008·Annual Review of Biophysics·Jayant B Udgaonkar
Aug 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kei-Ichi Okazaki, Shoji Takada
Aug 30, 2008·Science·Valeria VásquezEduardo Perozo
Oct 25, 2008·Nature·Evripidis GavathiotisLoren D Walensky
Dec 17, 2009·The Journal of Biological Chemistry·Stephanie BleickenEnrica Bordignon
Jul 31, 2010·Cell Death and Differentiation·M M ClelandR J Youle
Sep 11, 2010·Physical Chemistry Chemical Physics : PCCP·Tai-Ching Sung, Yun-Wei Chiang
Dec 1, 2010·Physical Chemistry Chemical Physics : PCCP·Yevhen PolyhachGunnar Jeschke
Jan 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·John McCoy, Wayne L Hubbell
May 27, 2011·The Journal of Physical Chemistry. B·Salvatore MagazùAntonio Benedetto
Aug 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Ya-Wen HuangYun-Wei Chiang
Sep 6, 2011·Physical Chemistry Chemical Physics : PCCP·Ya-Wen Huang, Yun-Wei Chiang
Sep 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mark R FleissnerWayne L Hubbell
Mar 13, 2012·Annual Review of Physical Chemistry·Gunnar Jeschke
Mar 20, 2012·Nature Materials·Ken-ichiro Murata, Hajime Tanaka
Jul 18, 2012·Nature·Shiou-Ru Tzeng, Charalampos G Kalodimos
Mar 15, 2013·Physical Chemistry Chemical Physics : PCCP·Tona von HagensGunnar Jeschke
Oct 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Yun-Hsuan KuoYun-Wei Chiang
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Carlos J LópezWayne L Hubbell
Dec 21, 2013·Nature Reviews. Molecular Cell Biology·Peter E CzabotarJerry M Adams
Feb 8, 2014·Trends in Biochemical Sciences·Tudor MoldoveanuDouglas R Green
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Michael T LerchWayne L Hubbell
May 16, 2014·Nature·Olivier DussFrédéric H-T Allain
Sep 18, 2014·Proceedings of the National Academy of Sciences of the United States of America·Dana WestphalRuth M Kluck
Nov 21, 2014·Molecular Biology of the Cell·Laura A GilliesTomomi Kuwana
Dec 3, 2014·Molecular Cell·Stephanie BleickenEnrica Bordignon

❮ Previous
Next ❯

Citations

Jan 20, 2021·Cell Death and Differentiation·Chien-Lun HungYun-Wei Chiang
Nov 20, 2020·Proceedings of the National Academy of Sciences of the United States of America·Chieh-Chin LiYun-Wei Chiang
Dec 29, 2020·Journal of Molecular Biology·Pei-Hua LuNien-Jen Hu
Nov 14, 2020·Communications Biology·Yu-Jing LanYun-Wei Chiang
Sep 18, 2020·The Journal of Physical Chemistry Letters·Ruei-Fong TsaiYun-Wei Chiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.