Abstract
This study suggests that the validation of reference genes is important for the accurate evaluation of differential gene expression in age-related hearing loss (AHL). To identify the most stable reference gene in the auditory cortex and cochlea of young and old rats. Materials and methods. mRNA levels of some common reference genes such as beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin C, hypoxanthine phosphoribosyl-transferase and eukaryotic translation elongation factor (EF) were quantified in the auditory cortex and cochlea of young and old rats by quantitative RT-PCR, and then their stability was evaluated using GeNorm and Normfinder software. There were some variations in the expression of reference genes found in the auditory cortex and cochlea of rats, wherein EF and GAPDH were identified as the most stable reference genes in auditory cortex and cochlea, respectively. mRNA levels of Cu/Zn-superoxide dismutase (SOD), Mn-SOD, catalase and glutathione peroxidase in the auditory cortex and cochlea do not vary significantly in young and old rats, when they are normalized with EF or GAPDH, the valid reference genes, respectively. However, Cu/Zn-SOD and catalase levels differ significantly in the audito...Continue Reading
References
Aug 19, 2000·BioTechniques·T SuzukiD R Crawford
Oct 27, 2001·Acta Oto-laryngologica·H StaeckerT R Van De Water
Dec 1, 2001·Analytical Biochemistry·H K HamalainenR Lahesmaa
Jun 18, 2002·Ageing Research Reviews·Michael D SeidmanUma Bai
Aug 30, 2002·Journal of Molecular Endocrinology·S A Bustin
Aug 30, 2002·Thorax·E M GlareE H Walters
Nov 6, 2003·Hearing Research·Konstantina M Stankovic, Gabriel Corfas
Apr 17, 2004·Journal of Immunological Methods·I R PetersM J Day
Aug 4, 2004·Cancer Research·Claus Lindbjerg AndersenTorben Falck Ørntoft
Jun 14, 2005·Brain Research. Molecular Brain Research·Eisuke KoyaAugust B Smit
Jul 19, 2005·Neurobiology of Disease·Lut Van LaerGuy Van Camp
Aug 2, 2005·Hearing Research·Elizabeth M KeithleyKenneth R Johnson
Feb 4, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jie ChenRunsheng Ruan
Aug 22, 2006·Neurobiology of Aging·Hongyan JiangSu-Hua Sha
Apr 20, 2007·Experimental Gerontology·Daniela BuckiovaJosef Syka
Citations
Aug 26, 2014·Cell and Tissue Research·Vesna Radojevic, Daniel Bodmer
Dec 20, 2011·Behavioural Brain Research·Carina Anicet RuthschillingMárcia Giovenardi
Dec 21, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Qiang WanJehee Lee
Feb 4, 2012·Neurobiology of Aging·Chiemi TanakaDonald Henderson
Nov 12, 2015·PloS One·Joanne R Chapman, Jonas Waldenström
Oct 9, 2014·Einstein·Ana Carolina de MouraAna Beatriz Gorini da Veiga
Sep 15, 2015·PloS One·Pedro Melgar-RojasJosé M Juiz