Identifying stable reference genes for evaluation of antioxidative enzyme gene expression in auditory cortex and cochlea of young and old Fischer 344 rats

Acta Oto-laryngologica
Jie Chen, Runsheng Ruan

Abstract

This study suggests that the validation of reference genes is important for the accurate evaluation of differential gene expression in age-related hearing loss (AHL). To identify the most stable reference gene in the auditory cortex and cochlea of young and old rats. Materials and methods. mRNA levels of some common reference genes such as beta-actin, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), ubiquitin C, hypoxanthine phosphoribosyl-transferase and eukaryotic translation elongation factor (EF) were quantified in the auditory cortex and cochlea of young and old rats by quantitative RT-PCR, and then their stability was evaluated using GeNorm and Normfinder software. There were some variations in the expression of reference genes found in the auditory cortex and cochlea of rats, wherein EF and GAPDH were identified as the most stable reference genes in auditory cortex and cochlea, respectively. mRNA levels of Cu/Zn-superoxide dismutase (SOD), Mn-SOD, catalase and glutathione peroxidase in the auditory cortex and cochlea do not vary significantly in young and old rats, when they are normalized with EF or GAPDH, the valid reference genes, respectively. However, Cu/Zn-SOD and catalase levels differ significantly in the audito...Continue Reading

References

Aug 19, 2000·BioTechniques·T SuzukiD R Crawford
Oct 27, 2001·Acta Oto-laryngologica·H StaeckerT R Van De Water
Jun 18, 2002·Ageing Research Reviews·Michael D SeidmanUma Bai
Apr 17, 2004·Journal of Immunological Methods·I R PetersM J Day
Jun 14, 2005·Brain Research. Molecular Brain Research·Eisuke KoyaAugust B Smit
Jul 19, 2005·Neurobiology of Disease·Lut Van LaerGuy Van Camp
Aug 2, 2005·Hearing Research·Elizabeth M KeithleyKenneth R Johnson
Feb 4, 2006·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Jie ChenRunsheng Ruan
Aug 22, 2006·Neurobiology of Aging·Hongyan JiangSu-Hua Sha
Apr 20, 2007·Experimental Gerontology·Daniela BuckiovaJosef Syka

❮ Previous
Next ❯

Related Concepts

Related Feeds

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.

Related Papers

Clinica Chimica Acta; International Journal of Clinical Chemistry
Wenjing Ruan, Maode Lai
Journal of Gastroenterology and Hepatology
Ashish S Verma, Bernard H Shapiro
Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP
Jae-Sung RheeJae-Seong Lee
© 2022 Meta ULC. All rights reserved