Identifying the Coiled-Coil Triple Helix Structure of β-Peptide Nanofibers at Atomic Resolution

ACS Nano
Andrew J ChristoffersonIrene Yarovsky

Abstract

Peptide self-assembly represents a powerful bottom-up approach to the fabrication of nanomaterials. β3-Peptides are non-natural peptides composed entirely of β-amino acids, which have an extra methylene in the backbone, and we reported fibers derived from the self-assembly of β3-peptides that adopt 14-helical structures. β3-Peptide assemblies represent a class of stable nanomaterials that can be used to generate bio- and magneto-responsive materials with proteolytic stability. However, the three-dimensional structure of many of these materials remains unknown. To develop structure-based criteria for the design of β3-peptide-based biomaterials with tailored function, we investigated the structure of a tri-β3-peptide nanoassembly by molecular dynamics simulations and X-ray fiber diffraction analysis. Diffraction data was collected from aligned fibrils formed by Ac-β3[LIA] in water and used to inform and validate the model structure. Models with 3-fold radial symmetry resulted in stable fibers with a triple-helical coiled-coil motif and measurable helical pitch and periodicity. The fiber models revealed a hydrophobic core and twist along the fiber axis arising from a maximization of contacts between hydrophobic groups of adjacent ...Continue Reading

References

Nov 17, 2001·Chemical Reviews·R P ChengW F DeGrado
Sep 26, 2002·Journal of the American Chemical Society·Richard P Cheng, William F DeGrado
Apr 3, 2003·Journal of the American Chemical Society·Scott A HartAlanna Schepartz
Dec 31, 2003·Methods in Enzymology·András Fiser, Andrej Sali
Oct 13, 2005·Journal of the American Chemical Society·Daniel TrzesniakWilfred F van Gunsteren
Aug 15, 2006·Biophysical Journal·Nitin RathoreJuan J de Pablo
Feb 8, 2007·Journal of the American Chemical Society·Douglas S DanielsAlanna Schepartz
Mar 31, 2007·Science·Hang YinWilliam F DeGrado
Jul 16, 2008·The Journal of Chemical Physics·Clark A MillerJuan J de Pablo
Oct 3, 2008·Accounts of Chemical Research·Yun-Dong WuYi-Lei Zhao
Dec 3, 2008·The Journal of Chemical Physics·Claire L PizzeyNicholas L Abbott
Dec 19, 2008·Angewandte Chemie·Manuel M MüllerDonald Hilvert
Jun 24, 2009·The Journal of Physical Chemistry. B·Jagannath MondalArun Yethiraj
Jun 27, 2009·The Journal of Physical Chemistry. B·Fredy SussmanRamón J Estévez
Feb 16, 2010·The Journal of Chemical Physics·Jagannath MondalArun Yethiraj
Apr 9, 2010·Journal of the American Chemical Society·Geronda MontalvoWilliam F DeGrado
May 8, 2010·Journal of the American Chemical Society·Scott J ShandlerWilliam F DeGrado
Jun 4, 2010·Chemical Society Reviews·Charlotte A E Hauser, Shuguang Zhang
Aug 3, 2010·Chemical Society Reviews·Derek N Woolfson, Zahra N Mahmoud
Oct 16, 2010·Organic Letters·Ivan V KorendovychScott J Shandler
Jul 26, 2011·Journal of the American Chemical Society·Scott J ShandlerWilliam F DeGrado
Nov 30, 2011·The Journal of Physical Chemistry. B·Jagannath MondalArun Yethiraj
Feb 22, 2012·Science·T AidaS I Stupp
Mar 3, 2012·The Journal of Chemical Physics·Jagannath Mondal, Arun Yethiraj
Mar 30, 2012·Biophysical Journal·Michael McGovernJuan J de Pablo
Apr 25, 2012·Methods in Molecular Biology·Kyle L Morris, Louise C Serpell
Dec 21, 2012·The Biochemical Journal·Kyle L MorrisLouise C Serpell
Oct 15, 2013·Journal of Computational Chemistry·Zhixiong Lin, Wilfred F van Gunsteren
Sep 2, 2014·Journal of Chemical Information and Modeling·Lukács J NémethTamás A Martinek
Sep 3, 2014·The Journal of Organic Chemistry·Gangavaram V M SharmaAkella V S Sarma
Jan 7, 2015·Soft Matter·Kyle L MorrisLouise C Serpell
Oct 30, 2015·Nature Communications·Sunbum KwonHee-Seung Lee
Jun 10, 2014·Journal of Chemical Theory and Computation·Wei HuangWilfred F van Gunsteren
Nov 21, 2015·Chemistry & Biology·Romila D GopalanMarie-Isabel Aguilar
Dec 13, 2011·Journal of Chemical Theory and Computation·Alpeshkumar K MaldeAlan E Mark
May 10, 2011·Journal of Chemical Theory and Computation·Wei HuangWilfred F van Gunsteren
Mar 1, 2008·Journal of Chemical Theory and Computation·Berk HessErik Lindahl

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Citations

Mar 5, 2019·Frontiers in Chemistry·Ketav KulkarniMarie-Isabel Aguilar
Jul 28, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Samuele Rinaldi
Apr 17, 2020·Frontiers in Chemistry·Nathan HabilaMark P Del Borgo
Jun 18, 2020·Frontiers in Bioengineering and Biotechnology·Seema GuptaPradeep Kumar
Oct 13, 2020·Chemical Science·Imola Cs SzigyártóTamás Beke-Somfai
Jul 3, 2021·Biomolecules·Michele Di FoggiaArmida Torreggiani
Aug 19, 2021·Journal of Chemical Information and Modeling·Sachini P Kadaoluwa PathirannahalageAndrew J Christofferson
May 28, 2020·ACS Nano·Paul Mulvaney
Nov 11, 2021·ACS Applied Materials & Interfaces·Ketav KulkarniMark P Del Borgo

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