Identifying the fitness costs of a pyrethroid-resistant genotype in the major arboviral vector Aedes aegypti.

Parasites & Vectors
Lisa M RigbyGregor J Devine

Abstract

Effective vector control measures are essential in a world where many mosquito-borne diseases have no vaccines or drug therapies available. Insecticidal tools remain the mainstay of most vector-borne disease management programmes, although their use for both agricultural and public health purposes has resulted in selection for resistance. Despite this, little is known about the fitness costs associated with specific insecticide-resistant genotypes and their implications for the management of resistance. In Aedes aegypti, the primary vector of dengue, chikungunya, and Zika, the best-characterised resistance mechanisms are single-point mutations that protect the voltage-gated sodium channel from the action of pyrethroids. We evaluated the fitness cost of two co-occurring, homozygous mutations (V1016G and S989P) by back-crossing a resistant strain of A. aegypti from Timor-Leste into a fully susceptible strain from Queensland. The creation of the backcross strain allowed us to isolate these kdr mutations in an otherwise susceptible genetic background. In comparison to the susceptible strain, the backcrossed colony exhibited longer larval development times (5 days, P < 0.001), 24% fewer mosquitoes reached the adult stage (P = 0.005)...Continue Reading

References

Jan 1, 1992·Annual Review of Entomology·R W MerrittE D Walker
Jan 1, 1987·The American Journal of Tropical Medicine and Hygiene·D M WattsA Nisalak
Nov 25, 2003·Journal of Evolutionary Biology·S BedhommeY Michalakis
Jun 6, 2007·Journal of Medical Entomology·Alongkot Ponlawat, Laura C Harrington
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Aug 4, 2009·Genome Research·David H AlexanderKenneth Lange
Jun 10, 2011·Bioinformatics·Petr DanecekUNKNOWN 1000 Genomes Project Analysis Group
Sep 5, 2012·Pest Management Science·Adi Kliot, Murad Ghanim
Apr 2, 2013·Journal of Medical Entomology·Hiang Hao Chan, Jaal Zairi
Jul 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yuzhe DuKe Dong
Jul 5, 2013·PLoS Neglected Tropical Diseases·Nils Benjamin TjadenCarl Beierkuhnlein
Oct 14, 2014·PloS One·Rebecca C ChristoffersonChristopher N Mores
Dec 17, 2014·Parasites & Vectors·Lillian L Moller-JacobsMatthew B Thomas
Apr 2, 2015·PloS One·Malal M DiopCédric Pennetier
Oct 16, 2016·Pesticide Biochemistry and Physiology·Letícia B SmithJeffrey G Scott
Mar 14, 2017·Emerging Infectious Diseases·Christopher M RoundyNikos Vasilakis
May 4, 2017·Behavior Genetics·Wayne G RostantNina Wedell
Aug 22, 2017·Current Opinion in Insect Science·Richard H Ffrench-Constant, Chris Bass
Jan 1, 2014·Advances in Insect Physiology·Kristopher S SilverKe Dong
Aug 3, 2018·Parasites & Vectors·Ashley G CallahanAry A Hoffmann
Sep 4, 2018·BioMed Research International·Michelangelo AuteriAlessandra Torina
Nov 16, 2018·Nature·Benjamin J MatthewsLeslie B Vosshall
Feb 7, 2019·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Shinji KasaiTakashi Tomita
Oct 11, 2019·PLoS Neglected Tropical Diseases·Isabelle DusfourFabrice Chandre

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Methods Mentioned

BETA
restriction associated sequencing
genotyping

Software Mentioned

Image J
DDemux
Bowtie
Geneious
VCFtools
GraphPad Prism
PLINK
ADMIXTURE

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