Jul 1, 1989

Identifying the fluorescent body (F-body) with quinacrine mustard staining of canine

Jikken dobutsu. Experimental animals
Y IgarashiS Ogawa

Abstract

The fluorescent body (F-body) was identified with quinacrine mustard (Q-M) staining in spermatozoon and lymphocyte of canine. Well washed sperm suspension was treated with protease (125 mg/ml) or dispase (2000p. u./ml) and staining with Q-M (final dilution 50 micrograms/ml) for 15 min to 24 hr at 37 degrees C. The lymphocyte cultures from whole blood were prepared as routine human investigation. The chromosomal preparation made by air dry method was stained with Q-M (final dilution 0.5 to 50 micrograms/ml) after pretreatment of enzyme digestion. The examination using a reflected fluorescent microscope revealed that the same F-body in human was present in both spermatozoon (20.1-39.7%) and interphase of lymphocyte (0.37.2%) of male origin.

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Mentioned in this Paper

Y Chromosome
Whole Blood
Interphase
Lymphoid Cells
Sperm Cell
Fluorescence
Endopeptidases
Proteolytic Enzyme
Fluorescent stain
Acrichine Yperite

About this Paper

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