Identifying the Genes Regulated by AtWRKY6 Using Comparative Transcript and Proteomic Analysis under Phosphorus Deficiency

International Journal of Molecular Sciences
Li-Qin LiLi-Ming Lu

Abstract

Phosphorus (P) is an important mineral nutrient for plant growth and development. Overexpressing AtWRKY6 (35S:WRKY6-9) was more sensitive and wrky6 (wrky6-1) was more resistant under low Pi conditions. To better understand the function of AtWRKY6 under low phosphate stress conditions, we applied two-dimensional gel electrophoresis (2-DE) to analyse differentially expressed proteins in the shoots and roots between wild type, 35S:WRKY6-9 and wrky6-1 after phosphorus deficiency treatment for three days. The results showed 88 differentially abundant protein spots, which were identified between the shoots and roots of 35S:WRKY6-9 and wrky6-1 plants. In addition, 59 differentially expressed proteins were identified in the leaves and roots of 35S:WRKY6-9 plants. After analysis, 9 genes with W-box elements in their promoter sequences were identified in the leaves, while 6 genes with W-box elements in their promoter sequences were identified in the roots. A total of 8 genes were identified as potential target genes according to the quantitative PCR (QPCR) and two dimension difference gel electrophoresis, (2D-DIGE) results, including ATP synthase, gln synthetase, nitrilase, 14-3-3 protein, carbonic anhydrases 2, and tryptophan synthase. ...Continue Reading

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Citations

Feb 2, 2021·Frontiers in Genetics·Prabin TamangRobert S Brueggeman
Nov 14, 2021·Plant Cell Reports·Ye HuangLiang Chen
Nov 7, 2021·The Plant Journal : for Cell and Molecular Biology·Paula da Fonseca-PereiraWagner L Araújo

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Methods Mentioned

BETA
electrophoresis
transgenic
protein folding

Software Mentioned

Excel
Protein Pilot
EggNOG
TargetP1
DeCyder
SPSS14
Blast
Mascot

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