Identifying the integrated neural networks involved in capsaicin-induced pain using fMRI in awake TRPV1 knockout and wild-type rats

Frontiers in Systems Neuroscience
Jason YeeCraig F Ferris

Abstract

In the present study, we used functional MRI in awake rats to investigate the pain response that accompanies intradermal injection of capsaicin into the hindpaw. To this end, we used BOLD imaging together with a 3D segmented, annotated rat atlas and computational analysis to identify the integrated neural circuits involved in capsaicin-induced pain. The specificity of the pain response to capsaicin was tested in a transgenic model that contains a biallelic deletion of the gene encoding for the transient receptor potential cation channel subfamily V member 1 (TRPV1). Capsaicin is an exogenous ligand for the TRPV1 receptor, and in wild-type rats, activated the putative pain neural circuit. In addition, capsaicin-treated wild-type rats exhibited activation in brain regions comprising the Papez circuit and habenular system, systems that play important roles in the integration of emotional information, and learning and memory of aversive information, respectively. As expected, capsaicin administration to TRPV1-KO rats failed to elicit the robust BOLD activation pattern observed in wild-type controls. However, the intradermal injection of formalin elicited a significant activation of the putative pain pathway as represented by such a...Continue Reading

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Citations

Dec 4, 2015·NeuroImage·Saeedeh AmirmohseniEsther Pogatzki-Zahn
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Nov 13, 2020·Pharmacology & Therapeutics·Vipin AroraMan-Kyo Chung

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Methods Mentioned

BETA
transgenic

Software Mentioned

Ekam
SPM8
Medical Image Visualization and Analysis ( MIVA )

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