Identifying Therapeutic Targets for Sepsis Research: A Characterization Study of the Inflammatory Players in the Cecal Ligation and Puncture Model

Mediators of Inflammation
Sara NullensBenedicte Y De Winter

Abstract

During sepsis, disturbed gastrointestinal motility and increased mucosal permeability can aggravate sepsis due to the increased risk of bacterial translocation. To help identify new therapeutic targets, there is a need for animal models that mimic the immunological changes in the gastrointestinal tract as observed during human sepsis. We therefore characterized in detail the gastrointestinal neuroimmune environment in the cecal ligation and puncture (CLP) model, which is the gold standard animal model of microbial sepsis. Mice were sacrificed at day 2 and day 7, during which gastrointestinal motility was assessed and cytokines were measured in the serum and the colon. In the spleen, lymph nodes, ileum, and colon, subsets of leukocyte populations were identified by flow cytometry. Septic animals displayed an impaired gastrointestinal motility at day 2 and day 7. Two days post-CLP, increased serum and colonic levels of proinflammatory cytokines were measured. Flow cytometry revealed an influx of neutrophils in the colon and ileum, increased numbers of macrophages in the spleen and mesenteric lymph nodes, and an enhanced number of mast cells in all tissues. At day 7 post-CLP, lymphocyte depletion was observed in all tissues coinci...Continue Reading

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Citations

Nov 22, 2019·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Philip PlaekeBenedicte Y De Winter
Jul 23, 2020·Critical Care Medicine·Clifford S DeutschmanUNKNOWN Research Committee of the Surviving Sepsis Campaign
Jul 18, 2020·Intensive Care Medicine Experimental·Clifford S DeutschmanUNKNOWN Research Committee of the Surviving Sepsis Campaign
Feb 2, 2021·International Journal of Nephrology·Jonathan S Chávez-IñiguezGuillermo García-García
Mar 16, 2021·Frontiers in Immunology·Christian B BergmannCharles C Caldwell

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Methods Mentioned

BETA
flow cytometry
PCR
FCS

Software Mentioned

GraphPad Prism
SPSS
FCS Express

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