Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow

Oncotarget
Gabrielis KundrotasRegina Liudkeviciene

Abstract

Human bone marrow mesenchymal stem cells (hBM-MSCs) hold promise for treating incurable diseases and repairing of damaged tissues. However, hBM-MSCs face the disadvantages of painful invasive isolation and limited cell numbers. In this study we assessed characteristics of MSCs isolated from residual human bone marrow transplantation material and expanded to clinically relevant numbers at passages 3-4 and 6-7. Results indicated that early passage hBM-MSCs are genomically stable and retain identity and high proliferation capacity. Despite the chromosomal stability, the cells became senescent at late passages, paralleling the slower proliferation, altered morphology and immunophenotype. By qRT-PCR array profiling, we revealed 13 genes and 33 miRNAs significantly differentially expressed in late passage cells, among which 8 genes and 30 miRNAs emerged as potential novel biomarkers of hBM-MSC aging. Functional analysis of genes with altered expression showed strong association with biological processes causing cellular senescence. Altogether, this study revives hBM as convenient source for cellular therapy. Potential novel markers provide new details for better understanding the hBM-MSC senescence mechanisms, contributing to basic s...Continue Reading

Associated Datasets

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Citations

Aug 2, 2017·Antioxidants & Redox Signaling·Rosa VonoPaolo Madeddu
Jul 28, 2016·Stem Cells and Development·Adam NowakowskiBarbara Lukomska
Mar 28, 2017·Archivum Immunologiae Et Therapiae Experimentalis·Sabrina GiacoppoEmanuela Mazzon
May 16, 2018·Stem Cells International·Yuxin HuYuanyuan Xu
Jan 10, 2019·Oxidative Medicine and Cellular Longevity·Monia OrcianiAndrea Ciavattini
Aug 13, 2017·BMC Genomics·Ian H BellayrRaj K Puri
Mar 15, 2019·Journal of Nanobiotechnology·Gabrielis KundrotasRicardas Rotomskis
Oct 10, 2020·World Journal of Stem Cells·Qing-Shu MengXiao-Ting Liang
Feb 12, 2020·European Journal of Pharmacology·Agata HadryśJoanna Jazowiecka-Rakus
Nov 16, 2017·Experimental Cell Research·Danika KhongBiju Parekkadan

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Datasets Mentioned

BETA
GSE68933

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

miScript miRNA PCR Array
NIS
Elements
RT 2 Profiler PCR Array Data Analysis
Leica CytoVision®
SPSS
ImageJ

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