IDH1 mutation in human glioma induces chemical alterations that are amenable to optical Raman spectroscopy

Journal of Neuro-oncology
Ortrud UckermannMatthias Kirsch

Abstract

Mutations in the isocytrate dehydrogenase 1 (IDH1) gene are early genetic events in glioma pathogenesis and cause profound metabolic changes. Because this genotype is found in virtually every tumor cell, therapies targeting mutant IDH1 protein are being developed. The intraoperative administration of those therapies would require fast technologies for the determination of IDH1 genotype. As of today, there is no such diagnostic test available. Recently, infrared spectroscopy was shown to bridge this gap. Here, we tested Raman spectroscopy for analysis of IDH1 genotype in glioma, which constitutes an alternative contact-free technique with the potential of being applicable in situ. Human glioma samples (n = 36) were obtained during surgery and cryosections were prepared. IDH1 mutations were assessed using DNA sequencing and 100 Raman spectra were obtained for each sample. Analysis of Raman spectra revealed increased intensities in spectral bands related to DNA in IDH1 mutant glioma while bands assigned to molecular vibrations of lipids were significantly decreased. Moreover, intensities of Raman bands assigned to proteins differed in IDH1 mutant and IDH1 wild-type glioma, suggesting alterations in the protein profile. The selecti...Continue Reading

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Nov 10, 2018·Neuro-oncology·Elisa AquilantiPriscilla K Brastianos
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Oct 15, 2019·Neuro-oncology Advances·Laurent James LivermoreOlaf Ansorge
Feb 22, 2021·Journal of Neuro-oncology·Todd Hollon, Daniel A Orringer
Jun 28, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·M KopecH Abramczyk

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