IDH1R¹³²H decreases the proliferation of U87 glioma cells through upregulation of microRNA-128a

Molecular Medicine Reports
Quanmin NieYongming Qiu

Abstract

Mutations in isocitrate dehydrogenase 1 (IDH1) are found in >70% of secondary glioblastomas and lower-grade gliomas (grades II-III). Among the numerous phenotypic differences between IDH1 mutant and wild-type glioma patients, the most salient is an improved survival rate for patients with a mutation. MicroRNAs (miRNAs) are a class of small, non‑coding, single‑stranded RNAs that can negatively regulate gene expression at the post‑transcriptional level, predominantly by binding to the 3'‑untranslated region of their target mRNAs. The dysregulated expression of several miRNAs has been reported to modulate glioma progression; however, it is unclear whether mutations in IDH1 regulate glioma cell proliferation through miRNA dysregulation. In the present study, stable overexpression of IDH1WT or IDH1R132H was established in the U87 glioma cell line. It was found that IDH1R132H decreased cell proliferation of U87 glioma cells by inducing the expression of the miRNA miR‑128a. This process was dependent on the transcription factor hypoxia inducible factor‑1α (HIF‑1α), which binds to a hypoxia response element in the promoter of miR‑128a. Furthermore, miR‑128a negatively regulated the expression of B‑cell‑specific Moloney murine leukemia ...Continue Reading

References

Jul 26, 2005·Biochemical and Biophysical Research Communications·S A CiafrèM G Farace
Sep 26, 2007·Human Molecular Genetics·Cherie Blenkiron, Eric A Miska
Oct 16, 2007·Cancer Cell·Sophia W M BruggemanMaarten van Lohuizen
Feb 21, 2009·The New England Journal of Medicine·Hai YanDarell D Bigner
Jun 16, 2009·Biochemical and Biophysical Research Communications·Jana NovakovaJaroslav Michalek
Jul 29, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marc SansonJean-Yves Delattre
Mar 30, 2011·Annals of Neurology·Linda B C BraltenPim J French
Apr 13, 2011·PloS One·Sujaya SrinivasanKumaravel Somasundaram
Aug 30, 2011·Oncogene·T PapagiannakopoulosK S Kosik
Mar 9, 2012·Brain Tumor Pathology·Koichi Ichimura
Mar 24, 2012·PloS One·Zhu-mei ShiBing-Hua Jiang
Dec 22, 2012·Neuro-oncology·Tor-Christian Aase JohannessenRolf Bjerkvig
Jun 5, 2013·Neuro-oncology·Pierpaolo PeruzziJakub Godlewski
Aug 29, 2013·BioMed Research International·Shirin FarivarFarzaneh Jadali

❮ Previous
Next ❯

Citations

Jun 4, 2019·International Journal of Molecular Sciences·Geon-Hee KimJi-Hong Lim
Jan 19, 2019·Oncology Letters·Guozhang HuChang Li
Jan 20, 2019·Journal of Experimental & Clinical Cancer Research : CR·Xueran ChenZhiyou Fang
Aug 28, 2019·International Journal of Molecular Sciences·Andrea DianaDaniela Murtas
Oct 17, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Guo Gord ZhuMeera Hameed

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
immunoprecipitation
ChIP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.